Algae rich in selenium and polyunsaturated fatty acid, product and preparation method

A technology of unsaturated fatty acids and algae, applied in biochemical equipment and methods, unicellular algae, and microorganism-based methods, etc., can solve the problems of reducing biomass, the limitation of selenium-enriched algae, toxicity, etc., and achieve safe absorption and utilization. The effect of eliminating toxic side effects and gastrointestinal irritation

Inactive Publication Date: 2019-04-05
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing production method of selenium-enriched algae is to add selenium at the beginning of fermentation, but selenium usually has certain toxicity to algae, and directly adding selenium at the beginning of fermentation will seriously reduce the biomass and affect the yield of oil , in order to reduce the toxicity of selenium to algae, the maximum amount of selenium added is only 8mg / L, which limits the amount of selenium enrichment of algae

Method used

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  • Algae rich in selenium and polyunsaturated fatty acid, product and preparation method
  • Algae rich in selenium and polyunsaturated fatty acid, product and preparation method
  • Algae rich in selenium and polyunsaturated fatty acid, product and preparation method

Examples

Experimental program
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Embodiment 1

[0056] The preparation method of the selenium-enriched polyunsaturated fatty acid algae product provided by the present embodiment is as follows:

[0057] Strains: Schizochytrium sp. The preservation number is CCTCC NO: M2012494.

[0058] 1 seed activation culture:

[0059] The Schizochytrium mutant strain CCTCC NO:M2012494 was inoculated into the activation medium and cultivated at a temperature of 25-28°C, the shaking speed of the shaker was 200r / min, and the cultivation time was 48h to obtain a primary seed activation medium.

[0060] Used activation medium comprises: glucose 10g / L, sodium glutamate 20g / L, yeast extract 10g / L, sodium chloride 15g / L, magnesium sulfate 0.5g / L, pH natural.

[0061] 2 Seed expansion culture:

[0062] When the bacterial concentration of the seed activation culture solution reaches 8% (volume concentration), inoculate it into the expansion medium according to the 10% (volume ratio) inoculation amount and cultivate it. The cultivation temperatur...

Embodiment 2-18

[0090] The preparation method of embodiment 2-18 is basically the same as embodiment 1, and the main difference of each embodiment is shown in table 1.

[0091] Table 1

[0092]

[0093]

[0094] Examples 17 and 18 are CCTCC NO: M2012494 starting strain CCTCCNO: M2012074, and patent CN107523504A discloses the mutation screening process of CCTCC NO: M2012494 using CCTCC NO: M2012074 as the starting strain.

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Abstract

The invention discloses algae rich in selenium and polyunsaturated fatty acid, a product and a preparation method. The preparation method for the algae rich in selenium and polyunsaturated fatty acidcomprises the step of carrying out fermentation, specifically, adding selenium salt into a fermentation culture medium which is inoculated with an algae body. According to the preparation method, thecontent of selenium in the algae can be effectively increased by optimizing a fermentation process so as to prepare the algae rich in selenium and polyunsaturated fatty acid.

Description

technical field [0001] The invention relates to the technical field of selenium-enriched fermentation, in particular to a selenium-enriched polyunsaturated fatty acid algae, a product and a preparation method. Background technique [0002] The benefits of selenium to the human body: Selenium (Se) is determined by the World Health Organization and the Chinese Medical Association as the third largest micronutrient and health element that must be supplemented after iodine and zinc in the 21st century. It has anti-oxidation, anti-cancer, detoxification, and Improve immunity and so on. At present, most of the selenium-enriched algae on the market are selenium-enriched spirulina and chlorella, because these two algae have high tolerance to selenium, and selenium-enriched products of other algae are rarely seen. [0003] Polyunsaturated fatty acids (Polyunsaturated fatty acids, PUFA) refers to straight-chain fatty acids containing two or more double bonds and a carbon chain length...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38Y02A40/818
Inventor 李翔宇陆姝欢熊淑婷余超汪志明
Owner CABIO BIOTECH WUHAN CO LTD
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