Algae rich in selenium and polyunsaturated fatty acid, product and preparation method
A technology of unsaturated fatty acids and algae, applied in biochemical equipment and methods, unicellular algae, and microorganism-based methods, etc., can solve the problems of reducing biomass, the limitation of selenium-enriched algae, toxicity, etc., and achieve safe absorption and utilization. The effect of eliminating toxic side effects and gastrointestinal irritation
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Embodiment 1
[0056] The preparation method of the selenium-enriched polyunsaturated fatty acid algae product provided by the present embodiment is as follows:
[0057] Strains: Schizochytrium sp. The preservation number is CCTCC NO: M2012494.
[0058] 1 seed activation culture:
[0059] The Schizochytrium mutant strain CCTCC NO:M2012494 was inoculated into the activation medium and cultivated at a temperature of 25-28°C, the shaking speed of the shaker was 200r / min, and the cultivation time was 48h to obtain a primary seed activation medium.
[0060] Used activation medium comprises: glucose 10g / L, sodium glutamate 20g / L, yeast extract 10g / L, sodium chloride 15g / L, magnesium sulfate 0.5g / L, pH natural.
[0061] 2 Seed expansion culture:
[0062] When the bacterial concentration of the seed activation culture solution reaches 8% (volume concentration), inoculate it into the expansion medium according to the 10% (volume ratio) inoculation amount and cultivate it. The cultivation temperatur...
Embodiment 2-18
[0090] The preparation method of embodiment 2-18 is basically the same as embodiment 1, and the main difference of each embodiment is shown in table 1.
[0091] Table 1
[0092]
[0093]
[0094] Examples 17 and 18 are CCTCC NO: M2012494 starting strain CCTCCNO: M2012074, and patent CN107523504A discloses the mutation screening process of CCTCC NO: M2012494 using CCTCC NO: M2012074 as the starting strain.
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