Blakeslea trispora rich in selenium and carotenoids, carotenoids fermentation product and fermentation method of blakeslea trispora

A technology of Brassella mould, fermentation method, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of weak selenium conversion ability, screening and acclimation takes a lot of time and manpower, etc., and achieve safe absorption and utilization , Eliminate toxic side effects and gastrointestinal irritation

Active Publication Date: 2019-03-12
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Our research shows that B. trispora has a certain selenium-enriching ability and a high tolerance to chemical selenium, but the selenium conversion ability of common B. trispora is weak, and generally high selenium-enriching ability is desired The fungi need to be screened and domesticated to obtain selenium-enriched Bradleyella trispora, but screening and domestication takes a lot of time and manpower

Method used

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  • Blakeslea trispora rich in selenium and carotenoids, carotenoids fermentation product and fermentation method of blakeslea trispora
  • Blakeslea trispora rich in selenium and carotenoids, carotenoids fermentation product and fermentation method of blakeslea trispora
  • Blakeslea trispora rich in selenium and carotenoids, carotenoids fermentation product and fermentation method of blakeslea trispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The method for fermenting and preparing selenium-enriched and β-carotene-enriched Borealis trispora provided by this embodiment is as follows:

[0063] Bacterial species: the preservation number of Blakeslea trispora BT7251(+) is CCTCC NO:M2014378, and the preservation number of Blakeslea trispora BT7603(-) is CCTCC NO:M2014379.

[0064] 1 slant culture:

[0065] The spore suspensions of positive bacteria and negative bacteria of B. trispora were taken, respectively spread on PDA slant medium, and cultivated in a constant temperature incubator at 25° C. for 5 days.

[0066] The slant medium used includes (g / L): glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; its preparation method can be referred to as follows: cut potatoes into 1cm squares and add deionized water to boil for 30 minutes, After cooling, filter with four layers of gauze, take the filtered clear liquid and add glucose and agar powder.

[0067] 2 seed cultures:

[0068] Use an inoculation shove...

Embodiment 2-17

[0103] The preparation methods of Examples 2-17 are basically the same as those of Example 1, and the main differences of each example are shown in Table 1. The enrichment method used in Examples 12 and 13 is to collect the bacterium sludge after filtering the bacteria and then make the following Add selenium salt in one step and continue to cultivate and ferment;

[0104] The inspection results of each embodiment are shown in Table 2. Among them, the detection method of lycopene is the same as GB / T 22249-2008.

[0105] Table 1

[0106]

[0107]

[0108]

[0109] Note: The fermenters used in the examples (Examples 2-8, etc.) without enrichment operations are common pilot-scale fermenters. Of course, it is also feasible to use the enrichment fermenter described in Example 1 for fermentation without enrichment. The fermenter described in Example 1 is most suitable for molds with hyphae. "-" indicates that the corresponding ingredient has not been added.

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Abstract

The invention discloses blakeslea trispora rich in selenium and carotenoids, a carotenoids fermentation product and a fermentation method of the blakeslea trispora rich in selenium and carotenoids andrelates to the technical field of selenium-rich fermentation. The preparation method of the blakeslea trispora rich in selenium and carotenoids comprises a step of adding selenium salt into a fermentation culture medium inoculated with blakeslea trispora. The preparation method is capable of preparing the blakeslea trispora rich in selenium and carotenoids. Through the method, the condition of the fermentation method is optimized; the selenium salt is added in a process of culturing the blakeslea trispora; selenium is absorbed and utilized when growing the blakeslea trispora, so that seleniumis organically combined with proteins and polysaccharides in the blakeslea trispora and is converted into biological selenium; the content of selenium in the blakeslea trispora is increased.

Description

technical field [0001] The invention relates to the technical field of selenium-enriched fermentation, in particular to a selenium-enriched carotenoid-enriched Brassella trispora trispora, a carotenoid fermented product and a fermentation method. Background technique [0002] Selenium (Se) is determined by the World Health Organization and the Chinese Medical Association as the third most essential micronutrient health element after iodine and zinc in the 21st century. [0003] Carotenoids are a general term for a class of important natural pigments, which are commonly found in yellow, orange-red or red pigments of animals, higher plants, fungi, and algae. Carotenoids are the main source of vitamin A in the body, and also have anti-oxidation, immune regulation, anti-cancer, and anti-aging effects. [0004] Β-carotene (C40H56) is one of the carotenoids and is also an orange-yellow fat-soluble compound. It is the most ubiquitous and stable natural pigment in nature. β-carote...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P23/00C12R1/645
CPCC12N1/14C12P23/00C12N1/145C12R2001/645
Inventor 李翔宇余超陆姝欢熊淑婷汪志明
Owner CABIO BIOTECH WUHAN CO LTD
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