Blakeslea trispora rich in selenium and carotenoids, carotenoids fermentation product and fermentation method of blakeslea trispora
A technology of Brassella mould, fermentation method, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of weak selenium conversion ability, screening and acclimation takes a lot of time and manpower, etc., and achieve safe absorption and utilization , Eliminate toxic side effects and gastrointestinal irritation
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Embodiment 1
[0062] The method for fermenting and preparing selenium-enriched and β-carotene-enriched Borealis trispora provided by this embodiment is as follows:
[0063] Bacterial species: the preservation number of Blakeslea trispora BT7251(+) is CCTCC NO:M2014378, and the preservation number of Blakeslea trispora BT7603(-) is CCTCC NO:M2014379.
[0064] 1 slant culture:
[0065] The spore suspensions of positive bacteria and negative bacteria of B. trispora were taken, respectively spread on PDA slant medium, and cultivated in a constant temperature incubator at 25° C. for 5 days.
[0066] The slant medium used includes (g / L): glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; its preparation method can be referred to as follows: cut potatoes into 1cm squares and add deionized water to boil for 30 minutes, After cooling, filter with four layers of gauze, take the filtered clear liquid and add glucose and agar powder.
[0067] 2 seed cultures:
[0068] Use an inoculation shove...
Embodiment 2-17
[0103] The preparation methods of Examples 2-17 are basically the same as those of Example 1, and the main differences of each example are shown in Table 1. The enrichment method used in Examples 12 and 13 is to collect the bacterium sludge after filtering the bacteria and then make the following Add selenium salt in one step and continue to cultivate and ferment;
[0104] The inspection results of each embodiment are shown in Table 2. Among them, the detection method of lycopene is the same as GB / T 22249-2008.
[0105] Table 1
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[0108]
[0109] Note: The fermenters used in the examples (Examples 2-8, etc.) without enrichment operations are common pilot-scale fermenters. Of course, it is also feasible to use the enrichment fermenter described in Example 1 for fermentation without enrichment. The fermenter described in Example 1 is most suitable for molds with hyphae. "-" indicates that the corresponding ingredient has not been added.
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