Cross-type genome recombination sorting method for genetically modified food

A sorting method and genome technology, which is applied in the fields of transgenic technology, biology, and genome recombination, can solve the problems of high time responsibility and inability to meet the traits of genetically modified foods, etc., achieve good simplicity and universality, reduce time complexity, and shorten Effects of Genome Sequencing

Pending Publication Date: 2019-04-05
龙口味美思环保科技有限公司
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AI Technical Summary

Problems solved by technology

The existing genome recombination technology has a high degree of time responsibility, and due to the lack of solutions for recombination analysis and other technologies, several genes in the genome are located at the same position or other genes are omitted. Combining them can only produce a partial sequence of the genome. Not optimal sorting, unable to meet the demand for traits possessed by genetically modified foods

Method used

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  • Cross-type genome recombination sorting method for genetically modified food
  • Cross-type genome recombination sorting method for genetically modified food
  • Cross-type genome recombination sorting method for genetically modified food

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Experimental program
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Embodiment Construction

[0013] refer to figure 1 , the method of the present invention comprises the following steps:

[0014] A. Apply an inversion operation to the gene fragment to form a new chromosome, and obtain the optimal inversion sequence of the genome through insertion;

[0015] (3) Genetically modified food is artificially controlled genome sequence recombination. Genome recombination mainly includes: inversion, transposition, transposition, insertion, deletion, inversion and transposition operations are on a single chromosome, while transposition operations are on two chromosomes. Given a chromosome X={x 1 ,x 2 ,...,x i-1 ,x i ,...,x j ,x j+1 ,...,x n}, apply a reverse operation r(i,j) to the gene segment (x i ,x i+1 ,...,x j-1 ,x j ), form a new chromosome X'={x 1 ,x 2 ,...,x i-1 ,-x j ,...,-x i ,x j+1 ,...,x n}.

[0016] X

x 1

x 2

…

x i-1

x i

…

x j

x j+1

…

x n

X′

x 1

x 2

…

x i-1

-x j

…

-x i ...

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Abstract

The invention discloses a cross-type genome recombination sorting method for genetically modified food. The method comprises the main steps that a reverse operation is applied to a gene segment to form a new chromosome, and an optimal reverse sequence of a genome is obtained through insertion; a chromosome forest is established, an indirect minimum sub array tree is set, a genome displacement deleting distance formula is calculated, a displacement insertion distance formula is deduced, and interaction-type displacement sorting is conducted; a turning operation is conducted on a sequence of thegenome, the turning sequence distance is minimum through cracking, and the genome is turned and sorted. The method has good simplification performance and universality, the indirect minimum sub arraytree is set for interaction-type reversion, displacement and turning sorting, the polynomial precise algorithm of genome recombination is provided, the time complexity of the algorithm is lowered, the recombined genome array is shortened, and a source gene is converted into a target genome through least operation.

Description

technical field [0001] The invention relates to the field of genome recombination, transgenic technology and biology, in particular to a method for cross-type genome recombination and sequencing of genetically modified food. Background technique [0002] Genetically modified food uses gene recombination technology to reorder and combine the original gene order of the species to form a genome that we want to show certain traits. The existing genome recombination technology has a high degree of time responsibility, and due to the lack of solutions for recombination analysis and other technologies, several genes in the genome are located at the same position or other genes are omitted. Combining them can only produce a partial sequence of the genome. It is not an optimal sort, and cannot meet the requirements for the traits of genetically modified foods. Contents of the invention [0003] In order to solve the above problems, the purpose of the present invention is to provid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B30/10
Inventor 杨迎霞
Owner 龙口味美思环保科技有限公司
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