Method for rapidly determining purity of barley grain beta-glucan extract

Abstract

The invention provides a method for rapidly determining the purity of a barley grain beta-glucan extract. The method comprises the steps that after beta-glucan is hydrolyzed to oligosaccharide by lysoglycan, and then is hydrolyzed to glucose by beta-glucosidase; after a color developing solution is added for heating in a water bath, the value delta A of glucose at 510 nm is measured by a microplate reader; and the purity of beta-glucan is calculated from the glucose concentration. The method provided by the invention has the advantages of simple operation, less reagent usage, simple used instrument, short extraction time and high production and application value, and is suitable for the rapidly determining the purity of the barley grain beta-glucan extract in laboratories, quality inspection centers, functional food processing plants and the like.

Description

technical field [0001] The invention relates to a method for determining the purity of an extract, in particular to a method for rapidly determining the purity of barley grain beta-glucan extract. Background technique [0002] Barley grain β-glucan is an important source of dietary fiber, has very good nutritional and health functions, and is increasingly valued by people. β-glucan reduces the absorption of cholesterol and bile acids by increasing the viscosity of food entering the intestine and increasing the activity of 7α-hydroxylase. Taking a certain amount of β-glucan in each meal, the blood sugar and insulin index in the body will decrease significantly after a meal; in addition, β-glucan can also reduce the occurrence of some serious diseases, such as: type II diabetes , cardiovascular disease, and colon cancer. [0003] At present, the determination method of barley grain β-glucan content and purity is still above the original chemical method, the determination pro...

AI Technical Summary

Problems solved by technology

[0003] At present, the determination method of barley grain β-glucan content and purity is still above the original chemical method, the determination process is cumbersome, the number of single determination samples is small, and the efficiency is extremely low when there are many samples to be determined

However, this largely limits the research and development of barley's nutritious food and the selection and cultivation of new special-purpose barley varieties, which needs to be improved.