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Solid state fermentation reactor equipped with active support material

A bioreactor and support technology, applied in the field of fermentation, can solve the problems of low capillary conductivity of liquid and so on

Active Publication Date: 2019-04-16
Q控制力公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] A problem in solid-state fermentation bioreactors is that the larger the gas space of the reactor, the lower the capillary conductivity of the liquid

Method used

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  • Solid state fermentation reactor equipped with active support material
  • Solid state fermentation reactor equipped with active support material
  • Solid state fermentation reactor equipped with active support material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Preferred conditions for porous solid supports were tested using a mixture comprising vermiculite, perlite and cation exchange resin.

[0075] Table 1. Physical properties of the solid support from a mixture of vermiculite (40vol%, 2-4mm), synthetic cation exchange resin (40vol%, 0.7mm) and perlite (20vol%, 1-2mm)

[0076]

[0077] Table 1 shows that the largest pores did not produce large liquid-gas interfaces. Excessive particle size reduction rapidly produces low water conductivity. The size of the pores between the particles is between 1 / 4 and 1 / 2 the size of the particles.

[0078] If according to the Sabatier equation CO 2 +4H 2 ->CH 4 +2H 2 O, a bioreactor producing methane from hydrogen and carbon dioxide at an efficiency (power) of 10 W / l produces water at a rate of 1.35 g / h / l. This means a water downflow of 0.135 cm / h at the bottom of the bioreactor structure close to one meter high.

[0079] The water conductance values ​​in Table 1 indicate that su...

Embodiment 2

[0082] This example shows that liquid products are drained from a solid state fermentation reactor by gravity.

[0083] according to figure 1 A bioreactor was constructed from polypropylene sewage pipes (75 mm inner diameter, 500 mm height, 2 L working volume) as shown in . Fit the nylon inlet tube for gas into the upper part of the pipe. Regulate the gas supply with a mass flow controller. The bioreactor is equipped with outlet pipes installed in the lower part of the pipes for gaseous and liquid end product collection.

[0084] The upper part of the sewage pipe of the bioreactor is filled with a solid support. A solid support was created by mixing 6L 2-4mm vermiculite with 2L 2-5mm perlite and 2L cation exchange resin prior to filling. In addition, sources of N and P supplemented by other materials are added to this mixture to serve as sources of nutrients and trace elements for biological reactions. The bioreactor is inoculated with microbial ammonia oxidizing bacteria...

Embodiment 3

[0087] This example shows that solid product (cellular biomass) is drawn out of a solid state fermentation reactor by gravity. Specifically, in this example, microbial proteins were produced from methane using bioconversion in a solid-state fermentation reactor.

[0088] according to figure 1 A bioreactor was constructed from polypropylene sewage pipes (75 mm inner diameter, 500 mm height, 2 L working volume) as shown in . Fit the nylon inlet tube for gas into the upper part of the pipe. The gas feed mixture of methane and oxygen was regulated with a mass flow controller. The bioreactor is equipped with outlet pipes installed in the lower part of the pipes for collection of gaseous, liquid and solid end products (single-cell proteinaceous biomass).

[0089] The upper part of the sewage pipe of the bioreactor is filled with a solid support. A solid support was created by mixing 6L 2-4mm vermiculite with 2L 2-5mm perlite and 2L cation exchange resin prior to filling. Furthe...

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Abstract

The present invention relates to an active solid state fermentation bioreactor for producing gases, liquid(s) or solids from gaseous or gaseous and liquid starting materials and to a fermentation process using such reactor. The bioreactor according to the present invention comprises three major phases; a solid phase comprising the porous solid support, a liquid phase comprising liquid and a gaseous phase. The solid phase comprises of a porous solid support in which at least 20% of the pore volumes have a size resulting in a liquid suction of about 0.01 to about 0.1 bars if these pores are filled with liquid; the porous solid support is inoculated with desired micro-organisms; the volume of the gaseous phase is 20% to 60% of the volume of the bioreactor and the liquid phase is at least 20%of the reactor volume. The unsaturated capillary conductivity of filling / packing solid material of the bioreactor is at least 0.1 cm / h. The present invention provides a solid state fermentation bioreactor which enables a large gas-liquid interface and in which the filling material has a good capillary conductivity despite the unsaturated state.

Description

technical field [0001] The present invention relates to mobile solid-state fermentation bioreactors for the production of gases, liquids or solids from gaseous, or gaseous and liquid feedstocks, and to fermentation processes using said reactors. Background technique [0002] Solid state fermentation is emerging as a potential technology for the production of microbial products such as feed, fuel, food, industrial chemicals and pharmaceuticals. Solid state fermentation methods offer several advantages over liquid fermentation methods. Solid-state fermentation is carried out in solid-state reactors, which can naturally provide a large gas phase and at the same time form a continuous liquid that fills the pore system. Furthermore, fairly dense microbial growth on moist solid particles can be obtained on sufficient surface area, resulting in high fermentation efficiencies. In cases where the aim is to build low-cost bioreactors, with low maintenance costs due to the low unit p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/16C12M1/04C12M1/107C12N11/14C12P5/02
CPCC12M1/04C12M1/107C12M1/16C12N11/14C12M25/18C12N1/20C12P1/04C12P7/02C12P7/40C12M21/04C12P5/02
Inventor A·阿利塔洛M·尼斯卡宁E·奥拉
Owner Q控制力公司
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