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Varroa destructor invasion behavior analyzing device and method for analyzing invasion preference of varroa destructor to apis cerana and apis mellifera larvae

A technique for Varroa mites, behavioral analysis

Active Publication Date: 2019-04-19
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difference is that Varroa dissociation does not pose obvious harm to the original host but poses a fatal threat to the new host

Method used

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  • Varroa destructor invasion behavior analyzing device and method for analyzing invasion preference of varroa destructor to apis cerana and apis mellifera larvae
  • Varroa destructor invasion behavior analyzing device and method for analyzing invasion preference of varroa destructor to apis cerana and apis mellifera larvae
  • Varroa destructor invasion behavior analyzing device and method for analyzing invasion preference of varroa destructor to apis cerana and apis mellifera larvae

Examples

Experimental program
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Effect test

Embodiment 1

[0031] like figure 1 and figure 2 As shown, the Varroa mite invasion behavior analysis device of the present invention comprises a petri dish 1, and the petri dish 1 comprises a petri dish bottom 11 and a petri dish cover 12, on two mutually perpendicular diameters of the circular bottom surface of the petri dish bottom 11, There are 4 honeybee breeding platform bases 2 fixedly spaced from the center of the circle, the height of the side wall at the bottom of the petri dish 11 is greater than the height of the honeybee Wangwang platform base 2, and the beeswax 3 at the same height as the honeybee Wangwang platform base 2 is housed in the petri dish bottom 11, and the petri dish bottom 11 Holes 4 are arranged on the beeswax 3 at the center.

[0032] The preparation method of the Varroa mite invasion behavior analysis device comprises the following steps:

[0033] a) Container selection. A commercially available standard 60mm high borosilicate glass culture dish (diameter of...

Embodiment 2

[0041] Example 2: Analysis of Invasion Preference of Varroa Dessie to Worker Bee Larvae of Chinese Apis and Italian Apis

[0042] The Chinese honey bee Apis cerana cerana, referred to as the Chinese bee, is one of the subspecies of the Oriental honey bee; the Italian honey bee Apis mellifera ligustica, referred to as the Italian honey bee, is one of the subspecies of the Western honey bee.

[0043] 1) Methods and steps:

[0044] a) Collect 5-day-old early worker bee larvae:

[0045] A healthy bee colony and a healthy bee colony were randomly selected respectively. In the evening of the first day, a sub-spleen was taken from the selected middle bee colony and Italian bee colony respectively, and the spleen surface was covered with transparent cellophane, and all the sealed workers' hives in the framed area were marked, and then the combs were removed. Put it back into the original group. On the next morning (interval 12-15 hours), the spleens marked yesterday were taken out, a...

Embodiment 3

[0057] Example 3: Analysis of Invasion Preference of Varroa Dessie to the Larvae of the Drones of the Chinese Bee and the Italian Apis

[0058] The Chinese honey bee Apis cerana cerana, referred to as the Chinese bee, is one of the subspecies of the Oriental honey bee; the Italian honey bee Apis mellifera ligustica, referred to as the Italian honey bee, is one of the subspecies of the Western honey bee.

[0059] 1) Methods and steps:

[0060] a) Collect 5-day-old early drone larvae:

[0061] The method of operation is basically the same as that described in the corresponding method and steps of Example 1, except that the marked ones are drone hives. For each batch of experiments, 40 drone larvae of 5-day-old early Chinese bee (Italian bee) were needed.

[0062] b) Collection of adult female Varroa disses:

[0063] The operation method is the same as the corresponding method and steps in Example 1. Each batch of experiments required 20 Varroa disses. In order to ensure the...

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Abstract

The invention discloses a varroa destructor invasion behavior analyzing device and a method for analyzing invasion preference of varroa destructor to apis cerana and apis mellifera larvae. The varroadestructor invasion behavior analyzing device comprises a culture dish, which is composed of a culture dish bottom and a culture dish cover; on two diameter lines perpendicular to each other on the round bottom surface of the culture dish bottom are provided with four bee queen rearing cups spaced from the center of the round bottom surface at equal intervals, the height of the side wall of the culture dish bottom is higher than that of the bee queen rearing cups, the inside of the culture dish bottom is provided with beewax level to the bee queen rearing cups, and the beewax in the center ofthe culture dish bottom is provided with a slot. The varroa destructor invasion behavior analyzing device is low in manufacturing difficulty and rapid in manufacturing and accordingly can effectivelycontrol the production cost and facilitate generalized application. Besides, the main manufacturing material, namely the pure beewax, is high in plasticity, so that the varroa destructor invasion behavior analyzing device has the potential of being further modified and reutilized.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and in particular relates to a device for analyzing the invasion behavior of Varroa mite and a method for analyzing the invasion preference of Varroa mite to larvae of Chinese bees and Italian bees. Background technique: [0002] Varroa destructor is a major ectoparasite of the western honey bee Apis mellifera. Varroa parasitism seriously weakens the health level of bee colonies and is one of the important factors leading to the extinction of bee colonies worldwide. The Varroa mite originally parasitized the eastern honeybee Apis cerana, and since the host migrated to the western honeybee in the 1950s, it has rapidly expanded in new hosts. The difference is that Varroa dissalis does not pose obvious harm to the original host but poses a fatal threat to the new host. The study of basic biological issues such as invasion behavior and host preference of Varroa mite will provide a theoretical ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李文峰韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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