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A method for inducing adventitious buds to rapidly propagate orchid seedlings

A plant, fast technology, applied in the fields of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of tissue culture seedling utilization, and achieve the effect of high proliferation efficiency

Active Publication Date: 2022-04-19
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the current research is mainly focused on exploring the effects of hormone combinations on the induction of meristems, but the tissue culture seedlings themselves have not been used according to the plant characteristics of Orchidaceae. The combination of hormones induces meristemization of new plants, which will greatly improve the overall reproductive efficiency of the multiplication stage

Method used

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  • A method for inducing adventitious buds to rapidly propagate orchid seedlings
  • A method for inducing adventitious buds to rapidly propagate orchid seedlings
  • A method for inducing adventitious buds to rapidly propagate orchid seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Select the Bletilla striata variety, and after the explants are sterilized, obtain the tissue-cultured seedlings, take the 6-week-old tissue-cultured seedlings, keep the main root of 1.0-2.0 cm under the ultra-clean workbench condition, and remove the remaining peripheral roots; keep For the pseudobulb 1.0-1.5cm above the main root, remove the remaining stems and leaves; cut the pseudobulb cross-section, the thickness of each thin layer is 2.0-3.0mm, inoculate in MS+TDZ 3.0mg / L+NAA 1.0mg / On the induction medium of L, place it in a light culture room at 25±2°C for 15 days; transfer the obtained adventitious buds to the medium of MS+6-BA 2.0mg / L+NAA 1.0mg / L , placed in a light culture room at 25±2°C for 5 weeks, the proliferation rate of adventitious buds was 3.5.

[0035] Specific as figure 1 As shown, a is a white seedling prepared for the experiment, with an enlarged pseudobulb; b is an enlarged view of the pseudobulb; c is cutting the pseudobulb, and each thin layer...

Embodiment 2

[0037]Select robust yellow-flowered white and (Bletilla ochracea) varieties, after the explants are sterilized, obtain tissue-cultured seedlings, take 6-week-old tissue-cultured seedlings, keep the 1.0-2.0cm main root under ultra-clean workbench conditions, and remove the remaining peripheral roots; Keep the main root and the pseudobulb 1.0-1.5cm above, and remove the remaining stems and leaves; cut the pseudobulb transversely, each thin layer has a thickness of 2.0-3.0mm, and inoculate it with MS+TDZ 3.0mg / L+NAA 2.0mg / L induction medium, placed in a light culture room at 25±2°C for 15 days; transfer the obtained adventitious buds to the medium of MS+6-BA 3.0mg / L+NAA 0.5mg / L Placed in a light culture room at 25±2°C for 5 weeks, the proliferation rate of adventitious buds was 4.

Embodiment 3

[0039] Select healthy and strong garlic orchid (Pleione bulbocodioides), after the explants are sterilized, obtain tissue culture seedlings, take 10 weeks old tissue culture seedlings, keep the main root of 1.0-2.0 cm under ultra-clean workbench conditions, and remove the remaining peripheral roots; keep For the pseudobulb 1.0-1.5cm above the main root, remove the remaining stems and leaves; cut the pseudobulb transversely, the thickness of each thin layer is 2.0-3.0mm, inoculate in MS+TDZ 0.5mg / L+NAA 0.1mg / On the induction medium of L, place it in a light culture room at 25±2°C and cultivate it for 15 days; transfer the obtained adventitious buds to the medium of MS+6-BA 1.0mg / L+NAA 0.5mg / L , placed in a light culture room at 25±2°C for 8 weeks, the proliferation rate of adventitious buds was 5.

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Abstract

The invention discloses a method for inducing adventitious buds to rapidly propagate orchid seedlings, which comprises the following steps: sterilizing the surface of explants of orchids to obtain tissue culture seedlings; selecting robust tissue culture seedlings, and removing the peripheral root; remove the main stem, keep the root and the pseudobulb; cross-cut the pseudobulb, inoculate it on the induction medium, and cultivate it under light; transfer the adventitious buds to the proliferation medium, and cultivate it under the light. The present invention establishes a novel rapid propagation method for orchidaceae seedlings induced by pseudobulbs to induce adventitious buds. The seedlings are formed directly, and the propagation efficiency in the early stage is increased by 3-8 times compared with the traditional propagation. Combined with the propagation stage, the overall propagation effect is improved. Up to 9‑48 times.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture rapid propagation, in particular relates to a method for inducing adventitious buds to carry out rapid propagation of orchid plant seedlings. Background technique [0002] Orchidaceae is the second largest family of angiosperms, which integrates medicinal and ornamental value, and has extremely high economic, ecological and scientific value. The fruit of Orchidaceae is generally a capsule, the number of seeds is very large but small, without endosperm, the germination rate of the seeds is extremely low in the natural environment, even if there is no necessary congenital conditions such as symbiotic fungi after germination, it is difficult to germinate into seedlings. Coupled with factors such as human activities and climate warming, the survival status of most wild orchids is not optimistic, and it is of urgent practical significance to increase protection of orchids. [0003] As a c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 唐志康王仁睿黄晶侯大斌余马曾明颖樊莉刘金焕陈梨
Owner SOUTHWEAT UNIV OF SCI & TECH
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