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Four types of Chinese cabbage hybrid purity identification core primers and hybrid purity indel molecular marker identification kit

A technology of hybrid purity and core primers, which is used in recombinant DNA technology, biochemical equipment and methods, and the determination/inspection of microorganisms.

Active Publication Date: 2022-04-12
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

But so far there is no universally applicable primer combination that can be used for the purity identification of Chinese cabbage hybrids

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  • Four types of Chinese cabbage hybrid purity identification core primers and hybrid purity indel molecular marker identification kit
  • Four types of Chinese cabbage hybrid purity identification core primers and hybrid purity indel molecular marker identification kit
  • Four types of Chinese cabbage hybrid purity identification core primers and hybrid purity indel molecular marker identification kit

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[0057] It should be noted that the following detailed description is exemplary and intended to provide further explanation of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.

[0058] It should be noted that the terminology used herein is only for describing specific embodiments, and is not intended to limit the exemplary embodiments according to the present disclosure. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations and / or combinations thereof.

[0059] In order to enable those skilled in the art to understand the technical solution of the present disclosure more clearly, the technical solu...

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Abstract

This disclosure relates to four types of Chinese cabbage hybrid purity identification core primers and hybrid purity InDel molecular marker identification kits. Through extensive screening of related InDel molecular markers, a group of hybrids with higher heterozygosity in different types of Chinese cabbage hybrids were excavated. High primer combinations, these primer combinations are used to identify the parents of corresponding types of Chinese cabbage, and the characteristic DNA fingerprints of the parents can be constructed, and the purity of the hybrid can be identified by using the primers or primer combinations with polymorphism between the parents. Combining these primers to form a kit greatly reduces the cost of self-developed markers by enterprises or breeding units, and has broad application prospects.

Description

technical field [0001] The disclosure belongs to the field of identification of agricultural vegetable varieties, in particular to core primers for identification of purity of four types of Chinese cabbage hybrids and an InDel molecular marker identification kit for the purity of hybrids. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] The cultivation area of ​​Chinese cabbage ranks first among all kinds of vegetables in my country, and the amount of seeds used is huge. At present, hybrids are mainly used in the production of Chinese cabbage. The purity of hybrid seeds is one of the important indicators of seed quality, and has an important impact on the commerciality and yield of Chinese cabbage. Shandong is the main supply area for Chinese cabbage hybrids, supplying 10 Chinese cabbage hybrids to all parts of the country every year. 5 kg, acco...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 刘栓桃赵智中张志刚王荣花王立华李巧云
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI