Use of a mixture of monosulfonic acid tetrazolium salt and PMS derivatives for microbial detection and its detection method

Abstract

The invention belongs to the technical field of medical biological detection, and in particular relates to the use and method of using monosulfonic acid tetrazolium salt for microbial detection; the mixture reagent of novel monosulfonic acid tetrazolium salt and PMS derivatives is non-toxic and can be combined with microorganisms. The characteristics of co-incubation and color change are used to monitor bacteria in real time, providing a rapid detection method for bacterial properties; the detection reagent uses EZMTT monosulfonic acid tetrazolium salt detection system, which shows a good linear dose relationship in the number of viable bacteria; The reagent passes the absorbance value of the monosulfonic acid tetrazolium salt reactant at 450nm to obtain the EC 50 The curve was used to evaluate the bacterial viability.

Description

A kind of mixture of monosulfonic acid tetrazolium salt and PMS derivative is used for microbial detection The use of the test and its detection method technical field The invention belongs to medical biological detection technology field, relate in particular to by monosulfonic acid tetrazolium salt and PMS derivative Use and method of a mixture for the detection of microorganisms. Background technique [0002] Mycobacterium tuberculosis (M. tuberculosis), commonly known as Mycobacterium tuberculosis, is a pathogenic bacterium that causes tuberculosis. infringing All organs of the body, but tuberculosis is the most common. Tuberculosis remains an important infectious disease to this day. An estimated one-third of the world's population infected with Mycobacterium tuberculosis. About 8 million new cases occur each year, and at least 3 million people die from the disease, according to WHO. my country built Before the founding of the People's Republic of China, th...

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Problems solved by technology

[0012] Living cells contain NAD(P)H, so tetrazolium salts are often used for detection, but traditional MTT, CCK8, WST8, MTS methods are toxic to cells and can only be detected for a few hours

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