Application of miR-1246 and/or TERF2IP in diagnosis and treatment of glioma

A technology of mir-1246, 1.mir-1246, applied in the application field of miR-1246 and/or TERF2IP in the diagnosis and treatment of glioma, can solve problems such as poor prognosis

Active Publication Date: 2019-05-17
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Despite advances in molecular understanding of glioma and advances in surgical techniques, radiotherapy, and chemotherapy, the prognosis of patients with glioma, especially glioblastoma (GBM), a WHO grade IV glioma, remains poor , with a median survival time of less than 15 months after initial diagnosis

Method used

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  • Application of miR-1246 and/or TERF2IP in diagnosis and treatment of glioma
  • Application of miR-1246 and/or TERF2IP in diagnosis and treatment of glioma
  • Application of miR-1246 and/or TERF2IP in diagnosis and treatment of glioma

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Experimental program
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Embodiment

[0061] 1. Method

[0062] 1.1 Cell culture

[0063] Human GBM cell lines U87MG, U251, A172 and human monocyte cell line U937 were obtained from the Cell Bank of the Chinese Academy of Sciences. The primary human glioblastoma cell line P3 was kindly provided by Prof. Rolf Bjerkvig. U87MG and U251 were cultured in DMEM [10% fetal bovine serum (FBS), and U937 was maintained with RPMI-1640 (10% FBS). The P3 cell line was maintained in neurostromal medium containing 2 mM GlutaMAX and B-27 (1×), penicillin / streptomycin (1×), 20 ng / ml epidermal growth factor and type 2 fibroblast growth factor. All cells were cultured in media supplemented with 10% fetal bovine serum. 100U / ml penicillin and 100mg / ml streptomycin at 37°C in 5% CO 2 Incubate in a humidified atmosphere with 95% air. To induce differentiation into macrophages, U937 cells (1×10 6 ) were incubated with 100 ng / ml PMA (Sigma, MO, USA) for 24 hours. All cell lines were identified by short tandem repeat (STR) analysis an...

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Abstract

The invention provides application of miR-1246 and/or TERF2IP in diagnosis and treatment of glioma. The fact that that microRNA-1246 (miR-1246) is the most abundant microRNA expressed in glioma-derived exosomes (GDEs) and is significantly up-regulated in anoxic glioma-derived exosomes (H-GDEs) is disclosed for the first time. In addition, the miR-1246 is also enriched with exosomes isolated from cerebrospinal fluid (CSF) of a patient with preoperative glioblastoma (GBM), and the exosomes miR-1246 in cerebrospinal fluid of the patient with GBM after tumor resection are significantly reduced. The MicroRNA-1246 is shown to have the strongest capability to induce polarization of M2 macrophages. In addition, studies find that H-GDEs-induced M2 macrophage polarization is mediated by the miR-1246/TERF2IP/STAT3 and miR-1246/TERF2IP/NF-kappa B pathways.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and molecular biology, and specifically relates to the application of miR-1246 and / or TERF2IP in the diagnosis and treatment of glioma. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Gliomas are the most common and aggressive primary tumors in the central nervous system, accounting for more than 80% of malignant brain tumors. Despite advances in molecular understanding of glioma and advances in surgical techniques, radiotherapy, and chemotherapy, the prognosis of patients with glioma, especially glioblastoma (GBM), a WHO grade IV glioma, remains poor , with a median survival time of less ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00
Inventor 李刚薛皓王劭博钱明禹张平赵荣荣
Owner SHANDONG UNIV QILU HOSPITAL
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