Kasp molecular marker of broad-spectrum rice blast resistance gene pi9 and its detection method and application
A molecular marker, rice blast resistance technology, applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of long breeding cycle and reduced phenotypic selection efficiency
Active Publication Date: 2022-03-29
南宁维尔凯生物科技有限公司
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Problems solved by technology
[0004] Traditional backcross breeding of insect-resistant rice varieties relies on phenotypic selection of rice. Environmental conditions and genotypes will reduce the efficiency of phenotypic selection, and the breeding cycle is long
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[0023] In order to describe the present invention in detail, the following examples are used to further illustrate. The experimental methods used in the examples are conventional methods unless otherwise specified, and the materials and reagents used are commercially available unless otherwise specified.
[0024] Through high-throughput sequencing of the whole genome of seven rice varieties (1205, RH, 91, 58, 1209, 1208, 1298), the sequencing results were compared with the rice genome published on NCBI to obtain the original SNP sites, and then The original SNP sites are screened out by methods such as experimental reliability filtering, and the SNP sites of the present invention with better polymorphisms are screened out. Specifically: the molecular marker of rice blast resistance broad-spectrum gene pi9, its SNP sites include SNP site 1 and SNP site 2, which are respectively located at base 10389084 or 10389610 of rice chromosome 6, and the corresponding molecular markers T...
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The invention discloses a molecular marker of rice blast resistance broad-spectrum gene pi9. The SNP sites of the molecular marker include SNP site 1 and SNP site 2, which are respectively located at base 10389084 or base 10389610 of chromosome 6 of rice. The corresponding molecular marker polymorphisms are C / T, G / C, respectively. Based on this, combined with KASP technology, the inventor also designed a corresponding detection primer set and established a corresponding detection method. The invention can efficiently detect two SNP molecular markers of the broad-spectrum rice blast resistance gene pi9, and they have high specificity in detecting the pi9 gene locus; the pi9 gene in rice varieties can be quickly identified by using the KASP detection technology, and the operation is convenient and the detection result is accurate Reliable, suitable for the identification and assisted breeding of rice pi9 gene, which solves the problems of low efficiency of traditional breeding and long breeding cycle.
Description
technical field [0001] The invention belongs to the technical field of plant molecular markers, and in particular relates to a KASP molecular marker of rice blast resistance broad-spectrum gene pi9, a detection method and application thereof. Background technique [0002] Rice (Oryza sativa L.) is the staple food for more than half of the world's population. Rice blast, also known as rice fever or fire blast, is a rice disease caused by blast fungus, which can cause up to 35% to 50% loss in production every year. Breeding new disease-resistant varieties of rice by using high-quality resources of rice disease-resistant genes is the most effective, economical and environmentally safe method for controlling rice blast. Therefore, it is of great significance to analyze the genetic loci of blast resistance in rice germplasm and excavate excellent resistance gene resources for breeding disease-resistant rice varieties. [0003] Up to now, more than 100 main blast resistance gene...
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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 何新柳韦娇君玉宁卢东长城何发俭
Owner 南宁维尔凯生物科技有限公司