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Application of combination of tripterine and erastin to medicine for treating non-small-cell lung cancer

A technology of erastin and non-small cell lung cancer, which is applied in the field of medicine, can solve the problems of little research, and achieve the effect of being suitable for large-scale production, inducing cell death, and low cost

Active Publication Date: 2019-05-21
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, how to improve the anti-tumor effect of tripterine within a biologically safe dose has become a research hotspot, but there is little research on the effect and mechanism of tripterine on erastin-induced ferroptosis

Method used

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  • Application of combination of tripterine and erastin to medicine for treating non-small-cell lung cancer
  • Application of combination of tripterine and erastin to medicine for treating non-small-cell lung cancer
  • Application of combination of tripterine and erastin to medicine for treating non-small-cell lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Effect of erastin on viability of human non-small cell lung cancer cells detected by CCK-8 assay

[0026] Experimental principle

[0027] CCK-8 reagent contains WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfone Acid phenyl)-2H-tetrazole monosodium salt) is a compound similar to MTT in the presence of the electron coupling reagent 1-methoxy-5-methylphenazinium dimethyl sulfate (1-Methoxy PMS) Under certain conditions, it can be reduced to a highly water-soluble orange-yellow formazan product Formazan by the dehydrogenase in the mitochondria (reference figure 1 ), the amount of Formazan produced is proportional to the number of viable cells. Therefore, this feature can be used to directly analyze cell proliferation and toxicity. The more and faster the cell proliferation, the darker the color; the greater the cytotoxicity, the lighter the color. Use a microplate reader to measure its absorbance at a wavelength of 450nm, and the amount...

Embodiment 2

[0035] CCK-8 method to detect the effect of tripterine on the viability of human non-small cell lung cancer cells

[0036] Experimental principle: with embodiment 1

[0037] Experimental Instruments and Materials

[0038] Cell culture incubator (Thermo Fisher), ELX-800 microplate reader (BioTek), inverted microscope (LEICADAMIRB), fetal bovine serum (Biological Industries), DMEM (high glucose) medium (GIBCO), CCK-8 (MCE), DMSO (Sigma), tripterine (Sigma), 96-well cell culture plate (NEST), non-small cell lung cancer cells (HCC827 cells, Shanghai Institute of Cells, Chinese Academy of Sciences).

[0039] Experimental procedure

[0040] Non-small cell lung cancer cells were seeded in a 96-well plate at a density of about 8,000 cells / well, and cultured in a cell culture incubator at 37°C, 5% CO2, and DMEM high-glucose medium containing 10% fetal bovine serum. When the cell density reached 70-80%, tripterine with gradient final concentrations of 0, 0.625, 1.25, 2.5, 5, 10, and ...

Embodiment 3

[0044] Effect of erastin and tripterine on viability of human non-small cell lung cancer cells detected by CCK-8 assay

[0045] Experimental principle: with embodiment 1

[0046] Experimental Instruments and Materials

[0047] Cell culture incubator (Thermo Fisher), ELX-800 microplate reader (BioTek), inverted microscope (LEICADAMIRB), fetal bovine serum (Biological Industries), DMEM (high glucose) medium (GIBCO), CCK-8 (MCE), DMSO (Sigma), erastin (Selleck), tripterine (Sigma), 96-well cell culture plate (NEST), non-small cell lung cancer cells (HCC827 cells, Shanghai Institute of Cells, Chinese Academy of Sciences).

[0048] Experimental procedure

[0049] Non-small cell lung cancer cells were seeded in a 96-well plate at a density of about 8,000 cells / well, and cultured in a cell culture incubator at 37°C, 5% CO2, and DMEM high-glucose medium containing 10% fetal bovine serum. When the cell density reaches 70-80%, add 2.5 μM erastin and gradient final concentrations of 0...

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Abstract

The invention discloses application of a combination of tripterine and erastin to medicine for treating a non-small-cell lung cancer. Experiments verify that tripterine of 1-5 microns and erastin of 1.25-5 microns are combined to treat cells of the non-small-cell lung cancer, so that the cells of the cancer are remarkably induced to die, the survival rate of the cells of the cancer is decreased, accordingly, the effect of treating the lung cancer is realized, and an important conception is provided for clinical treatment of the lung cancer.

Description

technical field [0001] The invention relates to the application of tripterine combined with erastin in the medicine for treating non-small cell lung cancer, which belongs to the field of medicine. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world, and it has become the first cause of death from malignant tumors in urban population in my country. Non-small cell lung cancer includes squamous cell carcinoma (squamous cell carcinoma), adenocarcinoma, and large cell carcinoma. Compared with small cell carcinoma, its cancer cells grow and divide more slowly, and spread and metastasize relatively later. Non-small cell lung cancer accounts for about 80% of all lung cancers, and about 75% of the patients are in the advanced stage when they are discovered, and the 5-year survival rate is very low. [0003] In 2012, scientists discovered a new mode of cell death that depends on iron ions, called ferroptosis. Ferroptosis is different fro...

Claims

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Application Information

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IPC IPC(8): A61K31/517A61K31/56A61P35/00
Inventor 谭克樊玉梅刘铭
Owner HEBEI NORMAL UNIV
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