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Formaldehyde-free tissue fixing preservative liquid and preparation method thereof

A technology for tissue fixation and antiseptic solution, which is applied to the field of formaldehyde-free tissue fixation and antiseptic solution and its preparation, can solve the problems of long fixation time, cumbersome operation, endangering human health, etc., achieves good fixation effect and antiseptic effect, and simplifies and cumbersome. Operation steps, the effect of improving work efficiency

Active Publication Date: 2019-05-28
HARBIN GREEN SPECIMEN TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the pollution of the environment and the harm of human health by the traditional fixative antiseptic solution (formalin), the fixed slices need to be repaired and processed, which is cumbersome and wastes precious time for pathological diagnosis. Moreover, the existing new fixative solution has a long fixation time and low fixation effect. Poor, change the morphological structure of tissue cells, the color of fixed tissue is not good, and also need to be repaired, long-term preservation of tissue or tissue specimens are easy to corrupt, etc.

Method used

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  • Formaldehyde-free tissue fixing preservative liquid and preparation method thereof
  • Formaldehyde-free tissue fixing preservative liquid and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This embodiment provides a formaldehyde-free tissue fixation antiseptic solution composition, which consists of 2-methyl-1,2-benzisothiazol-3-one (MBIT), dimethyl sulfoxide, ethanol, azone, 5 - Chloro-2-methyl-4-isothiazolin-3-one (CIT) and 2-methyl-4-isothiazolin-3-one (MIT), KNO 3 , KCl, CaCl 2 , isopropanol, propylene glycol, glycerin, vitamin C and purified water; the specific weight of each component is as follows:

[0030] 2-methyl-1,2-benzisothiazol-3-one (MBIT) 1.0kg, dimethyl sulfoxide 3kg, azone 2kg and ethanol 35kg (41kg protein fixative in total);

[0031] 5-Chloro-2-methyl-4-isothiazolin-3-one (CIT) 0.03kg and 2-methyl-4-isothiazolin-3-one (MIT) 0.01kg (0.04kg preservative in total);

[0032] Potassium nitrate 0.3kg, potassium chloride 0.3kg and calcium chloride 0.3kg (a total of 0.9kg osmotic pressure regulator);

[0033] Isopropyl Alcohol 3kg, Propylene Glycol 3kg and Glycerin 3kg (total 9kg moisturizer);

[0034] 0.1kg vitamin C;

[0035] 8kg of wat...

Embodiment 2

[0040] This embodiment provides a formaldehyde-free tissue fixation antiseptic solution composition, which consists of 2-methyl-1,2-benzisothiazol-3-one (MBIT), dimethyl sulfoxide, azone, ethanol, KCl 、NaNO 3 , sucrose, 2-methyl-4-isothiazolin-3-one (MIT), 5-chloro-2-methyl-4-isothiazolin-3-one (CIT), isopropanol, propylene glycol, polyethylene glycol Alcohol, vitamin C and purified water; the specific weight of each component is as follows:

[0041] 2-methyl-1,2-benzisothiazol-3-one (MBIT) 5.0kg, dimethyl sulfoxide 8kg, azone 5kg and ethanol 65kg (a total of 83kg protein fixative);

[0042] 5-Chloro-2-methyl-4-isothiazolin-3-one (CIT) 0.1kg and 2-methyl-4-isothiazolin-3-one (MIT) 0.03kg (a total of 0.13kg of preservatives);

[0043] Sodium nitrate 1.2kg, sodium chloride 1.2kg and sucrose 1.2kg (total 3.6kg osmotic pressure regulator);

[0044] 8kg of isopropanol, 8kg of propylene glycol, 15g of polyethylene glycol and 8kg of glycerin (total 39kg of moisturizer);

[0045] ...

Embodiment 3

[0051] This embodiment provides a formaldehyde-free tissue fixation antiseptic liquid composition, which consists of 2-methyl-1,2-benzisothiazol-3-one (MBIT), dimethyl sulfoxide, azone, ethanol, NaNO 3 , sucrose, NaCl, 2-methyl-4-isothiazolin-3-one (MIT), 5-chloro-2-methyl-4-isothiazolin-3-one (CIT), isopropanol, propylene glycol, poly Ethylene glycol, vitamin C and water; the specific weight of each component is as follows:

[0052] 2-methyl-1,2-benzisothiazol-3-one (MBIT) 2kg, dimethyl sulfoxide 7kg, azone 3kg and ethanol 55kg (a total of 67kg protein fixative);

[0053] 5-chloro-2-methyl-4-isothiazolin-3-one 0.06kg and 2-methyl-4-isothiazolin-3-one 0.02kg (0.08kg preservative in total);

[0054] Sodium nitrate 0.5kg, sodium chloride 1kg and sucrose 1kg (a total of 2.5kg osmotic pressure regulator);

[0055] 5kg of isopropanol, 5kg of propylene glycol, 10g of polyethylene glycol (20kg humectant in total);

[0056] 0.2kg vitamin C;

[0057] 10.22 kg of water.

[0058] Th...

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Abstract

The invention discloses formaldehyde-free tissue fixing preservative liquid and a preparation method thereof, and belongs to the technical field of tissue fixing liquid. The fixing preservative liquidis prepared from the following components in parts by weight: 41-83 parts of a protein fixing agent, 0.04-0.12 part of a preservative, 0.9-3.6 parts of an osmotic pressure adjusting agent, 9-39 partsof a humectant, 0.1-0.5 part of an antioxidant and 8-30 parts of water, wherein the protein fixing agent is composed of 2-methyl-1,2-benzisothiazolin-3-one, dimethyl sulfoxide and ethanol; and the preservative is composed of 5-chloro-2-methyl-4-isothiazoline-3-ketone and 2-methyl-4-isothiazoline-3-ketone. The fixing preservative liquid has the advantages of good fixation performance, strong preservative ability, no formaldehyde, and safty and no toxicity, and is suitable for tissue or specimen preservation, specimen fixation before pathological analysis and fixed storage of samples collectedby surgery.

Description

technical field [0001] The invention relates to a formaldehyde-free tissue fixation antiseptic solution and a preparation method thereof, belonging to the technical field of tissue fixation antiseptic solutions. Background technique [0002] In the field of medical pathological diagnosis, the traditional fixed preservation solution of tissues or tissue sections is formaldehyde solution (10% formalin). Although formaldehyde has strong hardening effect on tissue fixation, strong permeability and good bactericidal effect, its shortcomings are also obvious. The strong irritating smell and the easy volatilization and diffusion of formaldehyde have brought great obstacles to the follow-up treatment and observation of tissues by medical workers. It not only affects work efficiency, but also seriously affects human health. It is the cause of lung disease and lung cancer. The real culprit, at the same time, the strong irritating smell makes the work efficiency of medical staff low, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 夏东辉王金涛
Owner HARBIN GREEN SPECIMEN TECH DEV
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