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Application and product of dsRNA in potato beetle prevention and control

A potato beetle and insecticide technology, applied in DNA/RNA fragmentation, application, recombinant DNA technology and other directions, can solve the problems of immature dsRNA technology, high production cost, short shelf life, etc., and achieve mature production technology and low cost. , the effect of long shelf life

Pending Publication Date: 2019-05-28
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current RNAi technology still has obvious deficiencies: ① The dsRNA or siRNA that triggers RNAi is easily degraded by ubiquitous nucleases in the environment, so it is unstable; short

Method used

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  • Application and product of dsRNA in potato beetle prevention and control
  • Application and product of dsRNA in potato beetle prevention and control
  • Application and product of dsRNA in potato beetle prevention and control

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0030] Embodiment 1: The application of dsLdRpn6 in the control of potato beetle in this embodiment, wherein the nucleotide sequence of dsLdRpn6 is shown in SEQ ID NO:1.

specific Embodiment approach 2

[0031] Embodiment 2: The difference between this embodiment and Embodiment 1 is that dsLdRpn6 is used as the active ingredient of the potato beetle insecticide. Others are the same as the first embodiment.

specific Embodiment approach 3

[0032] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that: the bacterial liquid containing the genetically engineered bacteria expressing dsLdRpn6 is used as the potato beetle insecticide. Others are the same as the first or second embodiment.

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Abstract

The invention provides application and a product of dsRNA in potato beetle prevention and control, and relates to genetic products in potato beetle prevention and control and derivative products. ThedsRNA product has the advantages of strong stability, mature production technology, low cost and long shelf life, and is an effective and inexpensive 'genetic pesticide' product with high specificityand environmental protection. The application of the dsRNA product provided by the invention in potato beetle control has great and far-reaching significance for potato beetle prevention and control.After a potato beetle insecticide provided by the invention is sprayed to potato plant leaves with low dose, potted potato plants can continue to prevent pests for 28 days or longer, and the control effect reaches 80% or above; the potato beetle insecticide provided by the invention still has a strong poisoning effect on potato beetles under an outdoor strong ultraviolet condition It, the field prevention and control lasting is about 3 weeks, and the control effect gradually decreases from 50% to about 10%.

Description

technical field [0001] The invention relates to the application of a gene product in the control of potato beetle and its derivative product. Background technique [0002] Potato is an important grain, vegetable, feed crop and industrial raw material crop in the world. In recent years, due to its obvious comparative economic benefits and other characteristics, the planting area in my country has continued to increase, and it has become the first in my country after rice, wheat and corn. 4 major staple food crops. The potato beetle, Leptinotarsa ​​decemlineata, also known as the potato beetle, its English name Colorado Potato Beetle (CPB), belongs to the order Coleoptera and the Chrysolithidae. It is recognized as a destructive pest of potatoes worldwide, and it is also one of the major quarantine targets in my country. Its host range is relatively narrow, and it mainly harms more than 20 species of Solanaceae plants such as potatoes, eggplants, cultivated tomatoes, Heliconia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/16A01P7/04C12N15/113C12N15/70C12N1/21C12R1/19
Inventor 付开赟郭文超丁新华吐尔逊·阿合买提何江李超关志坚黄红梅郭秀梅木拉提·塔里木别克
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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