High temperature-resistant Pleurotus ostreatus and screening method and applications thereof
A kind of technology of Pleurotus pellagra and screening method, which is applied in the field of Pleurotus pellagra, can solve the problems of poor quality, lack of high-temperature type Pleurotus pellagra strains, shortage of quantity of Pleurotus pellagra, etc., and achieve good growth and good tolerance. Effects of high temperature capability and strong oxidation resistance
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[0050] The experimental methods in the following examples, unless otherwise specified, are conventional methods, and can be consulted from the methods described in biochemistry and related books.
[0051] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified. The reagents are of chemical purity and no manufacturer is designated.
[0052] In the phosphate buffer, solution A is disodium hydrogen phosphate, and solution B is sodium dihydrogen phosphate.
[0053] In the following examples, the biochemical incubator used is Shanghai Yuejin SPX-150-II, the shaker is Tianjin Uno HNY-2102, and the spectrophotometer is the Universal Ultraviolet Spectrophotometer, Tu-1901.
[0054] The preparation method of PDA medium is as follows: Take 200g of peeled potatoes, cut into small pieces (potato pieces) and put them in a pot, add 980ml of water, heat to boiling on a heater and keep boiling for 30min, pour into a measuring cup, us...
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