Application of pleurotus abalonus protein extract to prevention and control of grape downy mildew

Abstract

The invention discloses an application of a pleurotus abalonus protein extract to prevention and control of grape downy mildew and provides an agent for preventing and controlling the grape downy mildew, and the agent contains propamocarb and the pleurotus abalonus protein extract; and a preparation method of the pleurotus abalonus protein extract comprises the steps: smashing pleurotus abalonus sporophores, then, carrying out water extraction, and collecting a water-soluble substance to obtain a water-soluble extract of pleurotus abalonus; precipitating proteins in the water-soluble extract of pleurotus abalonus by using ammonium sulfate, collecting a precipitate, and dialyzing water for precipitation to obtain the pleurotus abalonus protein extract. The EC50s of propamocarb and the pleurotus abalonus protein extract to grape downy mildew are respectively 0.21 mg / L and 0.05 mg / L, a synergistic effect is generated by mixing propamocarb and the pleurotus abalonus protein extract according to the mass ratio of 1:10, and the EC50 of the mixture to the grape downy mildew is 0.04 mg / L. The pleurotus abalonus protein extract has the advantage of reducing the usage amount of a chemical agent on the aspect of controlling and preventing grape downy mildew.

Description

technical field [0001] The invention relates to the prevention and treatment of grape downy mildew in the field of biotechnology, in particular to the application of oyster mushroom protein extract in the prevention and treatment of grape downy mildew. Background technique [0002] Grape downy mildew is a worldwide grape disease. Grape downy mildew mainly damages leaves, and can also infect young tissues such as new shoots and young fruits. When the leaves are damaged, translucent, water-soaked, small spots with unclear edges appear on the leaf surface at first, and then gradually expand into light yellow to yellow-brown polygonal lesions, with different sizes and shapes, and sometimes several lesions are connected together , forming large yellow-brown dry spots. When the air is humid, white moldy matter (spore peduncle and sporangia of pathogenic bacteria) is produced on the back of the lesion. The diseased spots are dry and brown, and the diseased leaves are easy to fal...

AI Technical Summary

Problems solved by technology

The long-term high-volume use of chemical pesticides in grape production has caused a series of problems, such as the increase in the resistance of pathogenic bacteria / diseases and insects, fruit farmers have to increase the use of pesticides, and the cost of pesticides has increased; pesticide residues in fruits have exceeded the standard, and the risk of environmental pollution has increased There are many problems such as non-target biological damage and chronic toxicity, so grape production urgently needs high safety, low residue, and pollution-free pest control technology

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