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Method for utilizing UVB irradiation method for inducing ginseng hair roots to produce ginsenoside

A technology of ginsenosides and ginseng, which is applied in the field of preparation of biologically active substances, can solve the problems of short supply of ginseng, complicated ginseng cultivation techniques, long growth cycle, etc., and achieve the effects of low production cost, improved controllability, and guaranteed activity and efficacy

Inactive Publication Date: 2019-06-25
广州翰江生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ginsenoside, as a main active ingredient in ginseng, has a low yield and has not yet been artificially synthesized. At present, ginseng mainly relies on field cultivation. However, the cultivation technology of cultivated ginseng is complicated and the growth cycle is long, so that the supply of ginseng is in short supply. In order to solve this problem and meet people's needs, it is common sense to increase the content of ginseng secondary metabolites in various ways, such as expanded culture in tissue culture medium reactors, fed culture, induction of hairy roots, addition of elicitors, etc.
[0004] At present, the use of hydrolyzed milk protein and methyl jasmonate, the synergistic effect of these two elicitors, has few precedents in the culture of adventitious roots of ginseng

Method used

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preparation example Construction

[0066] Preparation of active cells, obtaining single cells or small numbers of cell clusters as active cells;

[0067] Cleaning: put active cells on gauze, and then rinse with water to remove impurities in the culture medium and obtain clean active cells;

[0068] Dispersion: Disperse the clean active cells in glycerin to obtain active ginsenosides.

[0069] The preparation of the active cells, obtaining a single cell or a small number of cell clusters as active cells includes:

[0070] Cell selection: select cell clusters with better shape from the obtained callus;

[0071] Subculture: subculture the cell clusters with better morphology to obtain loose granular cell clusters, and the subculture times are 2 to 4 generations;

[0072] Suspension culture: move the loose granular cell mass to a shake flask filled with culture medium for shake flask culture to obtain free cells;

[0073] Screening: Filter free plant cells, and screen single cells or small cell clusters as activ...

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Abstract

The invention discloses a method for utilizing a UVB irradiation method for inducing ginseng hair roots to produce ginsenoside. The method comprises the steps of utilizing the UVB irradiation method for inducing cultivation of the ginseng hair roots; utilizing the induced hair roots as calli, and separating cells rich in the ginsenoside; making the ginsenoside. Active cells of the original-ecologyginsenoside cultivated by means of the preparing method serve as active matter, the active potency of the ginsenoside is guaranteed, the quality of the active matter of the ginsenoside can be maintained for a long term, and the active matter of the ginsenoside is also made into finished products; the preparing procedure is simplified, the active matter of the ginsenoside which is made into the finished products can be proportioned as required, and then the controllability of manually regulating products is improved. The method has the advantages that the operation method is simple, the production cost is low, and the method is applicable to large-scale production.

Description

technical field [0001] The invention relates to the technical field of preparation of bioactive substances, in particular to a method for inducing ginseng hairy roots to produce ginsenosides by using a UVB irradiation method. Background technique [0002] Ginseng is a perennial dicotyledonous plant of Araliaceae. It has the functions of enhancing immunity, preventing and treating cardiovascular diseases, protecting the liver, regulating blood sugar, delaying aging, enhancing learning and memory ability, and is often used in medicine, health care products and cosmetics industry . [0003] Ginsenoside, as a main active ingredient in ginseng, has a low yield and has not yet been artificially synthesized. At present, ginseng mainly relies on field cultivation. However, the cultivation technology of cultivated ginseng is complicated and the growth cycle is long, so that the supply of ginseng is in short supply. In order to solve this problem and meet people's needs, it is common...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00
Inventor 宋锡明
Owner 广州翰江生物科技有限公司
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