Method for utilizing UVB irradiation method for inducing ginseng hair roots to produce ginsenoside
A technology of ginsenosides and ginseng, which is applied in the field of preparation of biologically active substances, can solve the problems of short supply of ginseng, complicated ginseng cultivation techniques, long growth cycle, etc., and achieve the effects of low production cost, improved controllability, and guaranteed activity and efficacy
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[0066] Preparation of active cells, obtaining single cells or small numbers of cell clusters as active cells;
[0067] Cleaning: put active cells on gauze, and then rinse with water to remove impurities in the culture medium and obtain clean active cells;
[0068] Dispersion: Disperse the clean active cells in glycerin to obtain active ginsenosides.
[0069] The preparation of the active cells, obtaining a single cell or a small number of cell clusters as active cells includes:
[0070] Cell selection: select cell clusters with better shape from the obtained callus;
[0071] Subculture: subculture the cell clusters with better morphology to obtain loose granular cell clusters, and the subculture times are 2 to 4 generations;
[0072] Suspension culture: move the loose granular cell mass to a shake flask filled with culture medium for shake flask culture to obtain free cells;
[0073] Screening: Filter free plant cells, and screen single cells or small cell clusters as activ...
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