Swine-origin arcanobacterium pyogenes and application thereof
A technology of Cryptobacterium pyogenes and pig origin, applied in the field of microorganisms, can solve the problems of lack, lack of theoretical data, lack of preventive measures, etc., and achieve the effect of strong toxicity
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Embodiment 1
[0065] Example 1: Isolation and identification of Cryptobacter pyogenes
[0066] 1.1 Disease data collection
[0067] The disease material came from a pig farm in Pingxiang City, Jiangxi Province, which died of illness and nursery pigs. The sick pigs had high fever, asthma, food loss, emaciation, and weakness of limbs. The lungs of the sick pigs were collected after death (see figure 1 ), transport at low temperature, and store at -20°C.
[0068] 1.2 Preparation of medium
[0069] TSB liquid medium: 30 g of tryptone soybean broth (TSB, purchased from BD Difco, USA) was dissolved in 1000 mL of ultrapure water, sterilized at 121 ° C for 15 min, and 50 mL of fetal bovine serum (purchased from Hangzhou Sijiqing Company) was added , stored at 4°C for later use.
[0070] TSA solid medium: Dissolve 40 g of tryptone soy agar (TSA, purchased from BD Difco, USA) in 1000 mL of ultrapure water, sterilize at 121 °C for 15 min, add 50 mL of fetal bovine serum, and distribute in sterile p...
Embodiment 2
[0081] Example 2: Toxicity test of Cryptobacter pyogenes TPY JX18
[0082] 2.1 Infection test of Cryptobacterium pyogenes TPY JX18 on mice
[0083] The pathogenicity of C. pyogenes TPY JX18 was determined by female Kunming mice aged 4-5 weeks, and normal saline was used as a negative reference. The inoculation dose was 8×10 8 CFU / piece, 3×10 8 CFU / piece, 1×10 8 CFU / piece, 5×10 7 CFU / piece, 3×10 7 CFU / only, each dose group was inoculated intraperitoneally with 8 mice, and each mouse was inoculated with 0.5 mL of bacterial suspension. Observed for 7 days, the pathogenicity results of the strain to mice are shown in Table 2.
[0084] Table 2 Lethality of Cryptobacter pyogenes TPY JX18 to mice
[0085]
[0086] At the initial stage, the mice with the disease showed depression, curled up into a ball, and showed loss of appetite. The highest inoculation dose (8×10 8 CFU) caused abdominal suppuration of all inoculated mice, and all of them became ill and died within 3 day...
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