A kind of mutant type of human tumor suppressor gene pml and its application
A tumor suppressor gene, mutant technology, applied in the field of genetic engineering, can solve the problem that the effect of tumor suppressor gene has not yet been seen
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[0027]The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The embodiments are only used to explain the present invention and not to limit the protection scope of the present invention.
[0028](1) Identification of deer family positive selection genes
[0029]First, the genomes of deer family species including roe deer, white-lipped deer, reindeer, elk, white-tailed deer, small muntjac, red muntjac and black muntjac were assembled manually. The genomes of 8 deer species (roe deer, white-lipped deer, reindeer, elk, white-tailed deer, small muntjac, red muntjac and black muntjac) and 53 outgroup species were selected. The above-mentioned genomes were respectively compared to the goat reference genome (Capra hircus, ARS1) by LAST whole-genome comparison software to obtain pairwise genome comparison files. Then, through the multiz multiple sequence comparison software, the above pairwise comparison results are combined i...
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