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Acid degradation solution

A technology of degradation solution and acid degradation, which is applied in the field of acid degradation solution and the preparation of oritavancin intermediate A82846B, which can solve the problems of high production cost, increased production cost, and difficulties in separation and purification

Active Publication Date: 2019-06-25
JIANGSU SENRAN CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] It can be seen from the above that the structures of the three substances are very close, the separation and purification work is quite difficult, and the production cost is thus increased. The proportion of the components of A82846B in the fermentation broth (the proportion of A82846B in the three substances) will greatly affect the extraction yield. Rate
At present, there are few studies on A82846B in my country, and the fermentation unit and component ratio are relatively low, resulting in high production costs

Method used

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Examples

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Effect test

Embodiment 1

[0030] The preparation of embodiment 1 seed liquid

[0031] Seed medium: maltodextrin 20g / L, glucose 10g / L, soybean powder 15g / L, yeast extract 3g / L, calcium carbonate 1g / L, pH7.0.

[0032] Pack 150ml of seed culture medium into a 750ml Erlenmeyer bottle, and sterilize at 120°C for 30 minutes before use.

[0033] Inoculate the A82846B production strain Nocardia orientalis NRRL 18098 stored at low temperature into the prepared seed bottle, and shake the bottle for 40-50 hours at a temperature of 30°C and a rotation speed of 250 rpm to obtain mature shake bottle seeds.

Embodiment 2

[0035] Fermentation medium: maltodextrin 40g / L, molasses 20g / L, soybean powder 20g / L, yeast powder 10g / L, calcium carbonate 3g / L, calcium chloride 10g / L, pH7.0.

[0036] Pack 35L of fermentation medium in a 50L fermentation tank, and sterilize at 120°C for 30 minutes before use.

[0037] Inoculate 3.5L of mature shake flask seeds into a fermenter with 35L of feed liquid, culture conditions: temperature 30°C, ventilation volume 1VVM, tank pressure 0.05Mpa, initial speed 200rpm, and control the dissolved oxygen above 30% by adjusting the speed. Cultivate for 5 days to obtain a fermented liquid, and the fermentation unit is 515 mg / L.

Embodiment 3

[0039] The fermented liquid obtained in Example 2 was adjusted to pH=10.3 with NaOH solution, stirred for 2 hours, and plate-and-frame press-filtered to obtain 35 L of press-filtered liquid, which was adjusted to pH=9.2, and then introduced into LX18 adsorption resin. After purifying 9L of resin with water with a pH of 8, desorb with 8L of 1.0% acetic acid aqueous solution, and mix the components with a concentration greater than 500mg / L to form a primary desorption mixture, then perform nanofiltration on the desorption mixture, and concentrate to The unit is 35000mg / L.

[0040] The concentrated solution is introduced into the C18 column that has been balanced, and the eluent (5% acetonitrile: 0.5% NH 4 h 2 PO 4 =3:97 (v / v)) for elution, and the components with a purity of more than 90% were collected for extracting the product. A82846B purity below 90% is impurity waste liquid.

[0041] Collect the impurity waste liquid, concentrate it by nanofiltration, and concentrate unt...

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Abstract

The invention discloses an acid degradation solution for fermentative preparation of an oritavancin intermediate A82846B. A preparation method of the degradation solution includes extracting a fermentation culture solution obtained by fermenting and culturing amycolatopsis orientalis, conducting acid degradation on the extracted impurity waste solution to obtain the acid degradation solution and adding the acid degradation solution to a fermentation medium for fermentation again. By means of addition of impurity acid degradation solutions in different concentrations, the fermentation unit of A82846B is greatly improved, and the composition is significantly improved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation production, and relates to the fermentation of glycopeptide antibiotics, in particular to a method for preparing oritavancin intermediate A82846B by fermentation and an acid degradation liquid used as a synthetic precursor of A82846B. Background technique [0002] Glycopeptide antibiotics are a large class of substances produced by microorganisms, or produced and partially modified by microorganisms. Vancomycin and teicoplanin are commercially available antibacterial products. Among the glycopeptides discovered in the 1990s, there are those known as A82846A (also known as ereomomycin), A82846B (also known as chloroorienticinA), A82846C (also known as orienticinC) and orienticin A . A variety of modifications have been made to naturally occurring glycopeptides, one of which is the reductive alkylation of reactive amines in glycopeptides. [0003] On August 7, 2014, the FDA approve...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 袁建栋刘会明
Owner JIANGSU SENRAN CHEM
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