Application of mulberry leaf extract in inhibiting freshwater fish farming stress, feed, and preparation method of feed

A technology of mulberry leaf extract and freshwater fish, which is applied in the field of fish farming, can solve the problems of unreported application and achieve the effects of improving economic benefits, good extraction effect, and reducing morbidity and mortality

Active Publication Date: 2019-07-05
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmaceutical research results show that mulberry leaves have a variety of pharmacological activities, and have the functions of scavenging reactive oxygen species (ROS) in vivo and in vitro and chelating metal ions, but there is no application of mulberry leaf extracts in inhibiting the stress of freshwater fish farming. to report

Method used

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  • Application of mulberry leaf extract in inhibiting freshwater fish farming stress, feed, and preparation method of feed
  • Application of mulberry leaf extract in inhibiting freshwater fish farming stress, feed, and preparation method of feed
  • Application of mulberry leaf extract in inhibiting freshwater fish farming stress, feed, and preparation method of feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Mulberry leaf extract, its preparation method is as follows:

[0043] (1) 1000g of mulberry leaves are dried to constant weight in a blast drying oven at 50°C, and crushed through a 18-mesh sieve to obtain mulberry leaf powder;

[0044] (2) Take 400g of mulberry leaf powder, mix it according to the ratio of mulberry leaf powder and cyclohexane 1:6g / mL, stir at 600r / min for 6h, and obtain clarified filtrate and filter residue after filtration;

[0045] (3) According to step (2), the filter residue was extracted twice more, and the filtrates extracted three times were combined, and the filtrate was distilled under reduced pressure to constant weight to remove cyclohexane, so as to obtain the mulberry leaf cyclohexane extract.

Embodiment 2

[0047] Mulberry leaf extract, its preparation method is as follows:

[0048] (1) 1000g of mulberry leaves are dried to constant weight in a blast drying oven at 50°C, and crushed through a 18-mesh sieve to obtain mulberry leaf powder;

[0049] (2) Take 400g of mulberry leaf powder, mix according to the ratio of mulberry leaf powder and cyclohexane 1:6g / mL, stir at 600r / min for 6h, obtain the filter residue after filtration, and repeat the extraction of the filter residue twice;

[0050] (3) Mix the filter residue obtained in step (2) with diethyl ether at a ratio of 1:6g / mL, stir at 600r / min for 6h, filter to obtain a clarified filtrate and filter residue, repeat the extraction of the filter residue twice, and combine the filtrate extracted three times;

[0051] (4) The filtrate obtained in step (3) is distilled under reduced pressure to a constant weight to remove the ether to obtain the ether extract of mulberry leaves.

Embodiment 3

[0053] Mulberry leaf extract, its preparation method is as follows:

[0054] (1) 1000g of mulberry leaves are dried to constant weight in a blast drying oven at 50°C, and crushed through a 18-mesh sieve to obtain mulberry leaf powder;

[0055] (2) Take 400g of mulberry leaf powder, mix according to the ratio of mulberry leaf powder and cyclohexane 1:6g / mL, stir at 600r / min for 6h, obtain the filter residue after filtration, and repeat the extraction of the filter residue twice;

[0056] (3) Mix the filter residue obtained in step (2) with diethyl ether at a ratio of 1:6g / mL, stir at 600r / min for 6h, obtain the filter residue after filtration, and repeat the extraction of the filter residue twice;

[0057] (4) Mix the filter residue obtained in step (3) with acetone at a ratio of 1:6g / mL, stir at 600r / min for 6h, then filter the mixture to obtain a clear residue-free filtrate and filter residue, and repeat the extraction of the filter residue 2 times. Merge the filtrates extra...

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Abstract

The invention provides an application of mulberry leaf extract in inhibiting freshwater fish farming stress, a feed, and a preparation method of the feed. A preparation method of the mulberry leaf extract comprises: drying and crushing mulberry leaves, adding cyclohexane for extraction, filtering, extracting with ethyl ether, filtering, and distilling the filtrate under reduced pressure to constant weight. The invention further provides a ratio of the mulberry leaf extract in an anti-stress feed and the preparation method of the feed. By applying the mulberry leaf extract as an anti-stress agent in feeds, stress reaction of freshwater fishes, caused by high farming density, copper sulfate, dipterex and the like during farming can be inhibited, anti-stress capacity of the freshwater fishescan be improved, and disease incidence and mortality rate of the freshwater fishes can be decreased; novel uses of mulberry leaf can be developed, and mulberry leaf resources can be utilized effectively.

Description

technical field [0001] The invention belongs to the technical field of fish farming, and in particular relates to the application of mulberry leaf extract in suppressing the stress of freshwater fish farming, feed and a feed preparation method. Background technique [0002] High-density intensification is an important feature of current aquaculture. High-density breeding increases the feeding amount, increases the breeding waste in the water body, and deteriorates the water quality, manifested as a decrease in dissolved oxygen in the water, and excessive ammonia nitrogen and nitrite content. It has been reported that these factors can lead to oxidative stress damage in fish, thereby reducing the digestion and absorption of feed by fish, reducing the growth and immune function of fish, and increasing the morbidity and mortality of fish. High density farming also leads to excessive use of chemical disinfectants and pesticides. Copper sulfate is one of the most commonly used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A23K40/10
CPCA23K50/80A23K10/30A23K40/10Y02A40/818
Inventor 李华涛刘影吴敏马玉婷罗岚卢磊刘思妙冯敏
Owner NEIJIANG NORMAL UNIV
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