Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof

A chitosan nanometer, bronchial asthma technology, applied in the field of biomedicine, can solve the problems of inability to reduce airway reactivity, reduce airway sensitivity, etc., achieve easy grasp of operation methods, reduce airway sensitivity, and reduce airway sensitivity. Effects of Inflammatory Response

Active Publication Date: 2020-08-04
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it does not reduce airway responsiveness and airway sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof
  • A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof
  • A kind of composite chitosan nanoparticle for treating bronchial asthma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of DGKα gene and OVA antigen composite chitosan nanoparticles (CS-DGKα-OVA)

[0045] 1. Construct a vector expressing DGKα gene: synthesize the fusion gene of mouse IL-2 signal peptide sequence and mouse DGKα gene with full gene, and clone into pEGFP-N3 vector to obtain DGKα plasmid. Sequencing confirmed that the IL-2 signal peptide and mouse DGKα gene sequence were completely consistent with IL-2 (NM_008366.3) and DGKα gene (NM_016811.2) in GenBank. Because of the signal peptide sequence, after the DGKα gene is transfected into the cell, the cell can express the DGKα protein and secrete it outside the cell.

[0046] 2. Preparation of CS-DGKα-OVA:

[0047] 2.1 Dissolve chitosan (molecular weight: 100,000, DD: 95%) in 1% acetic acid aqueous solution to prepare a chitosan acetic acid solution with a concentration of 1.5 mg / mL, adjust the pH to 5.5-5.7 with NaOH, and sterile 0.22 μm Membrane filtration.

[0048] 2.2 Dissolve the DGKα plasmid in a sterile 0.22μm membr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biological medicine, in particular to composite chitosan nano particles for treating bronchial asthma and application thereof. The composite chitosan nano particles are particles formed by using CS (chitosan) to wrap DGKalpha gene(diacylglycerol kinase alpha gene) expression vectors and OVA (ovalbumin), can evidently lower OVA specific IgE antibody level in serum and bronchial inflammatory reaction and can evidently lower asthma airway responsiveness and airway sensitivity, and accordingly the particles are promising in application prospect in the preparation of drugs and / or health-care products for treating and / or preventing bronchial asthma.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a composite chitosan nanoparticle for treating bronchial asthma and its application. Background technique [0002] Bronchial asthma is a chronic airway allergic inflammatory disease characterized by the involvement of a variety of inflammatory cells and the infiltration of airway eosinophils. After the human body inhales allergens, the airway antigen presenting cells take up and present the allergens to T cells, which promotes the excessive activation and proliferation of T cells, which initiates the occurrence of asthma. Of which CD4 + Excessive activation of T cells is thought to play an important role in the pathogenesis of asthma. CD4 + After activation of T cells, the differentiation into Th2 cells increases, and the overactivation of Th2 cells leads to an immune response dominated by Th2 response, which eventually leads to the accumulation of inflammatory cells such as eosinophils in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/50A61K47/36A61P11/06A61K39/35
CPCA61K9/5036A61K39/35A61K48/0033A61P11/06A61K2300/00
Inventor 王彦毕玉田郭东霖黄丽云王斌
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com