Kits for the detection of respiratory pathogens in community-acquired pneumonia

Abstract

The invention discloses a kit for detecting respiratory pathogens of community-acquired pneumonia, and belongs to the technical field of biomedical detection. The kit comprises a reaction solution, aprimer solution, an enzyme solution, a negative quality control substance, a positive quality control substance, a hybrid color developing solution, a hybrid membrane strip and a desiccant, wherein aprobe is attached to the hybrid membrane strip, the hybrid color developing solution comprises microspheres connected with a second coloring marker, and the color is developed after the microspheres are enriched. The detection kit involves 24 common respiratory pathogens of CAP, and has the advantages of high specificity, high sensitivity, large number of detection targets, simple and rapid operation and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, in particular to a kit for detecting respiratory pathogens of community-acquired pneumonia. Background technique [0002] At present, the morbidity and mortality of infectious diseases have been high, among which respiratory tract infection ranks first among all kinds of infectious diseases. Community-acquired pneumonia (CAP) refers to the infectious lung parenchyma (including alveolar wall, that is, pulmonary interstitium in a broad sense) inflammation suffered outside the hospital, including pathogen infection with a clear incubation period. Pneumonia of internal onset. Globally, CAP is one of the most common respiratory infectious diseases that seriously threaten human health, with a wide range of transmission, high morbidity and high mortality. According to data from foreign CAP monitoring networks, the 30-day mortality rate of adult CAP patients is 8.6 %, the mortality rate of...

AI Technical Summary

Problems solved by technology

[0022] Due to the complex pathogenic spectrum and high incidence of CAP disease, there are very few nucleic acid detection products for multi-link detection. For the early diagnosis of CAP respiratory diseases, there are still many problems that need to be solved urgently: (1) The common respiratory pathogens of CAP are diverse. More than 20 common pathogens have been reported, and most cases are mixed infections, so the detection kit is required to realize the parallel detection of more than 20 pathogens without cross-interference between species, and has high sensitivity and specificity. There is no similar product or invention patent yet; (2) In addition to bacteria, common respiratory pathogens of CAP are mostly RNA viruses, DNA viruses, mycoplasma and chlamydia, etc., so the detection kit is required to have the ability to detect RNA and DNA nucleic acids at the same time, multi-indicator (3) Most CAPs are acutely onset and concentrated outbreaks, so the detection kits are required to have the ability of simple operation, rapid detection and high-throughput detection; (4) ) The variability of virus antigens is extremely strong, and the types of virus subtypes are increasing day by day, so the detection gene region, primers and probes of the detection kit are required to have good coverage and specificity

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