This invention discloses a disc-shaped
microfluidic chip detection kit and method, relating to the field of rapid
food safety detection. A disc-shaped substrate has a ring-shaped central flow channel, and in the direction of rotation towards the central flow channel, a
lysis buffer chamber, an
elution buffer chamber, a first washing buffer chamber, a second washing buffer chamber, and a
magnetic bead reagent chamber are arranged in a ring-shaped sequence. At least four reaction chambers are arranged in a ring-shaped interval in the direction away from the rotation axis of the central flow channel. A ring
magnet is positioned on the bottom plate facing the central flow channel, and a pin for opening a contact valve is arranged around the periphery of the ring
magnet. Two types of heating plates with temperature differences are respectively positioned on the heating plate facing the reaction chambers. This invention relies on
centrifugal force to achieve autonomous fluid drive, combined with constant temperature zone control to complete rapid amplification. It not only eliminates cross-
contamination problems caused by
magnetic bead transport at the
structural level, but also achieves a high degree of integration of the entire process from
nucleic acid extraction to
fluorescence amplification, effectively improving the overall detection efficiency of pathogens.