Tissue culture and rapid seedling growth method for Huai chrysanthemum

A technology of tissue culture and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of chrysanthemum virus accumulation, variety degradation, chrysanthemum yield and quality decline, etc., achieve short cycle, easy operation, low cost effect

Inactive Publication Date: 2019-07-12
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above situation, in order to solve the defects of the prior art, the purpose of the present invention is to provide a method for quickly raising seedlings of Chrysanthemum chrysanthemum tissue culture, which can effectively sol

Method used

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  • Tissue culture and rapid seedling growth method for Huai chrysanthemum
  • Tissue culture and rapid seedling growth method for Huai chrysanthemum
  • Tissue culture and rapid seedling growth method for Huai chrysanthemum

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Embodiment 2

[0030] 1) Acquisition of aseptic seedlings of chrysanthemum chrysanthemum: collect 2 cm chrysanthemum chrysanthemum terminal buds, sterilize with 0.1% mercuric chloride for 3 minutes, rinse with sterilized water 5 times, inoculate it on the first solid medium of 50 ml, and the inoculum size is 5 one, adjust the pH value to 5.8, and place it under the conditions of 23°C, relative humidity of 60%, light intensity of 1700lx, and light time of 12h·d-1 for cultivation;

[0031] 2) Tissue culture rapid propagation: cut the young leaves 0.5cm × 0.5cm of strong and strong Chrysanthemum chrysanthemum tissue culture seedlings, still inoculate it on the first solid medium of 50ml, the inoculum size is 5, make the first solid culture The pH value of the base is 5.8, and it will germinate after being cultivated in a sterile room for 5 days. After 18 days, the bud induction rate reaches 100%, and the multiplication factor can reach 9-10. 5, adjust the pH value to 5.8, and place them at 23°C...

Embodiment 3

[0035] 1) Acquisition of aseptic seedlings of chrysanthemum chrysanthemum: collect 3 cm terminal buds of chrysanthemum chrysanthemum, sterilize with 0.1% mercuric chloride for 3 minutes, rinse with sterilized water 6 times, inoculate it on the first solid medium of 50 ml, and the inoculum size is 5 one, adjust the pH value to 5.8, and place it under the conditions of 24°C, relative humidity of 60%, light intensity of 2000lx, and light time of 12h·d-1 for cultivation;

[0036]2) Tissue culture rapid propagation: cut the young leaves 0.5cm × 0.5cm of strong and strong Chrysanthemum chrysanthemum tissue culture seedlings, still inoculate it on the first solid medium of 50ml, the inoculum size is 5, make the first solid culture The pH value of the base is 5.8, and it will germinate after being cultivated in a sterile room for 5 days. After 18 days, the bud induction rate reaches 100%, and the multiplication factor can reach 9-10. 5, adjust the pH value to 5.8, and place them at 24...

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Abstract

The invention relates to a tissue culture and rapid seedling growth method for Huai chrysanthemum and is intended to effectively solve the problem that frequent use of continuous cropping in the maincurrent propagation methods, such as cuttage and suckering, causes accumulation of chrysanthemum viruses, resulting in variety degradation and decline in chrysanthemum yield and quality. According tothe solution, the tissue culture and rapid seedling growth method for Huai chrysanthemum comprises the steps of 1) acquiring sterile seedlings of Huai chrysanthemum; 2) carrying out tissue culture andrapid propagation; 3) hardening seedlings; 4) transplanting to a field. The tissue culture and rapid propagation technique is utilized herein to acquire sterile seedlings of Huai chrysanthemum in a tissue culture chamber; the sterile seedlings are multiplied before seedling strengthening, rooting, hardening, and transplanting; the whole production process has a short period, operation is convenient and simple, the multiplication fold is high, the cost is low, the acquired field seedlings have high survival rate, the leaves are deeply green, no thin, weak stems occur, the growth is good, and the tissue culture and rapid seedling growth method for Huai chrysanthemum is innovative.

Description

technical field [0001] The invention relates to the technical field of chrysanthemum cultivation, in particular to a rapid seedling raising method of chrysanthemum tissue culture. Background technique [0002] Huaiju, a perennial herb of Compositae, has high medicinal value and a long history. Huaiju tends to calm the liver and yang, so it got its name because it was produced in the ancient Huaiqing Mansion (now under the jurisdiction of Jiaozuo City, Henan Province). It is one of the famous "Four Huai Medicines". It can dispel wind and dissipate heat, detoxify. It has various pharmacological activities such as anti-oxidation, liver protection, anti-virus, and prevention of cardiovascular diseases. With the gradual development of traditional Chinese medicine, the demand for medicines for Huaiju has also increased greatly. [0003] Due to the current propagation methods such as cuttings and divisions are mainly used, and continuous cropping is often used, resulting in the ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 董诚明苏秀红邢冰刘雅琳娄玉霞魏硕
Owner HENAN UNIV OF CHINESE MEDICINE
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