Evaluation system and method for stress responses of sea horses
An evaluation method, hippocampus technology, applied in biochemical equipment and methods, microbial measurement/inspection, material inspection products, etc., can solve the problems of small range of activities, restricting the rapid development of seahorse factory breeding, and low swimming degree. To achieve the effect of promoting healthy development
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[0035] Taking enteritis induced by bacterial infection as an example, the stress response of the hippocampus was evaluated. The specific process included: sample preparation for bacterial infection of the hippocampus, detection of symptom intervals of 26 indicators and determination of corresponding scores, and determination of the degree of stress response of the hippocampus.
[0036] 1. Bacterial Infection Hippocampal Sample Preparation
[0037] A total of 120 5-month-old healthy hippocampi were selected. The average body weight and standard body length were (3.86±0.023) g and (9.58±0.037) cm, respectively. They were divided into four groups, and the male and female were cultured separately. Frozen mysid shrimp were fed as food three times a day, with residual food debris and faeces aspirated after each feeding for two hours.
[0038] The pathogenic strain (Edwardsiella tarda YT1) was diluted to 10 with sterile physiological saline (PSS) 5 cfu / mL, 10 7 cfu / mL, 10 9 cfu / m...
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