Evaluation system and method for stress responses of sea horses

An evaluation method, hippocampus technology, applied in biochemical equipment and methods, microbial measurement/inspection, material inspection products, etc., can solve the problems of small range of activities, restricting the rapid development of seahorse factory breeding, and low swimming degree. To achieve the effect of promoting healthy development

Inactive Publication Date: 2019-07-12
LUDONG UNIVERSITY
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AI Technical Summary

Problems solved by technology

In the past 10 years, the factory culture of seahorses in my country has developed rapidly, but because seahorses are quiet, have low swimming speed, small range of activities, and most of the time they cling to the attachment base, they are extremely sensitive to changes in environmental factors and human interference, so breeding is very difficult. Big
[0003] Moreover, during the breeding process of seahorses, it is very easy to produce stress responses to changes in temperature, salinity, pollutants, dissolved oxygen, pathogens, human operations and other factors, and even suffer from diseases, which will affect the breeding efficiency. Stress Response Detection and Evaluation System
However, the existing technology does not have an evaluation system in this regard, which makes it impossible for seahorse breeding practitioners to timely and objectively evaluate the impact of various factors on hippocampal immune stress, which seriously restricts the rapid development of seahorse factory farming.

Method used

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  • Evaluation system and method for stress responses of sea horses
  • Evaluation system and method for stress responses of sea horses
  • Evaluation system and method for stress responses of sea horses

Examples

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Embodiment 1

[0035] Taking enteritis induced by bacterial infection as an example, the stress response of the hippocampus was evaluated. The specific process included: sample preparation for bacterial infection of the hippocampus, detection of symptom intervals of 26 indicators and determination of corresponding scores, and determination of the degree of stress response of the hippocampus.

[0036] 1. Bacterial Infection Hippocampal Sample Preparation

[0037] A total of 120 5-month-old healthy hippocampi were selected. The average body weight and standard body length were (3.86±0.023) g and (9.58±0.037) cm, respectively. They were divided into four groups, and the male and female were cultured separately. Frozen mysid shrimp were fed as food three times a day, with residual food debris and faeces aspirated after each feeding for two hours.

[0038] The pathogenic strain (Edwardsiella tarda YT1) was diluted to 10 with sterile physiological saline (PSS) 5 cfu / mL, 10 7 cfu / mL, 10 9 cfu / m...

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Abstract

The invention discloses an evaluation system for stress responses of sea horses. The evaluation system is characterized by comprising 26 indexes in the five aspects of the outside index, immunocytochemistry, antibacterial peptide gene, immune factors and blood cells, symptom intervals of the 26 indexes and corresponding scores, total points and evaluation conclusions. The evaluation system and method for the stress responses of the sea horses involve the growth indexes, the physiological indexes and the immune indexes, and systematically evaluate the stress responses of the sea horses from behaviors, dissection and the molecular level, the indexes can detect the stress responses of the sea horses in different aspects and multiple levels, the evaluation system can reflect the trigger time,the response mode and the intensity of the stress, and the evaluation system can help people master stress response characteristics of sea horses to different stress factors.

Description

technical field [0001] The invention belongs to the technical field of animal stress response evaluation, and in particular relates to an evaluation system and evaluation method of hippocampal stress response. Background technique [0002] Seahorse (Hippocampus) is a national second-class protected animal and a designated protected animal under the International Washington Convention. It is an important aquarium ornamental animal and a precious Chinese medicinal material with extremely high economic value. In the long-term evolution process, seahorses have evolved unique physiological structures and behaviors. However, due to overexploitation of resources and severe damage to habitats, wild seahorses are on the verge of extinction. Therefore, artificial breeding of seahorses is becoming more and more important. In the past 10 years, the factory culture of seahorses in my country has developed rapidly, but because seahorses are quiet, have low swimming speed, small range of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/02G01D21/02G01N33/50G01N33/569G01N33/68
CPCC12Q1/6888C12Q2600/124C12Q2600/158G01D21/02G01N33/5002G01N33/5005G01N33/5094G01N33/56966G01N33/56972G01N33/6863
Inventor 王凯王晓蒙于智芬陈军房燕姜韩薛圆圆俞兰良张宁林强
Owner LUDONG UNIVERSITY
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