Environment-friendly antifreezing agent for fruit trees and preparation method of antifreezing agent
An antifreeze and environment-friendly technology, which is applied in the field of environment-friendly antifreeze for fruit trees and its preparation, can solve the problems of strong suppression of plant germination, growth and development of plants with reduced yield, and limited antifreeze effect, so as to increase the action time and prolong the effective antifreeze period , long-lasting antifreeze effect
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Embodiment 1
[0038] 1. Preparation of plant cell revitalizing agent nanoliposome solution:
[0039] 1) After ultrasonically dissolving 0.8 g soybean lecithin and 0.06 g β-sitosterol in chloroform, desolvate under reduced pressure at 20-30°C to obtain a smooth phospholipid film;
[0040] 2) Add 20 mg of seaweed polysaccharide, 20 mg of soybean peptide and 10 mg of surfactant PVP-K15 to the phospholipid film, then add 20 ml of pH 7.0 phosphate buffer, diffuse under ultrasonic conditions in a water bath, and then conduct ultrasonic diffusion in a cell ultrafine pulverizer Crush (working 2 s / gap 2 s, power 350 W, 20 min), and then centrifuge the obtained product (5°C, 6000 r / min, 10 min) to remove impurities in the lower layer, and finally filter with a 0.22 microporous membrane to obtain The filtrate is the plant cell revitalizing agent class nano liposome solution.
[0041] Two, prepare the nano-microcapsule dispersion of prohexadione calcium:
[0042] 1) 1.0g of prohexadione calcium was h...
Embodiment 2
[0054] The antifreeze performance test is carried out to the environment-friendly antifreeze agent emulsion for fruit trees prepared by embodiment 1 and its comparative examples 1 and 2. With the red Fuji apple tree (8 years old) in Zhaoyuan area, Yantai as the test relative, in the apple flowering period (4 In the middle and late months of the month) the weather forecast has a frost forecast in the early morning of April 26 (only on that day). Spray the entire crown of the apple tree around noon on the 25th (one antifreeze agent is used for every 100 trees); the lowest temperature from the night of April 25 to the morning of the 26th is -3.2°C, and at noon on April 28, the top and middle of each tree crown are counted. A total of 90 petals that have been unfolded next to each other, and the flower whose flower vessel turns light brown or brown is the damaged flower (if the damaged flower ≤ 1 among the 90 petals, it is regarded as not frozen, and 2≤ the damaged flower ≤ 5 is re...
Embodiment 3
[0060] The antifreeze performance test of the environment-friendly antifreeze emulsion for fruit trees prepared in Example 1 and Comparative Examples 1 and 2 was carried out with the pear tree (6 years old) in Laiyang, Yantai as the test phase. There was a cooling forecast in the early morning of March 9, 2009, and the environment-friendly antifreeze emulsion for fruit trees prepared in Example 1 and Comparative Examples 1 and 2 (15-20kg per 20g of water) was used to treat the pear tree as a whole around noon on March 03. Spraying (use one antifreeze agent for every 100 trees); the lowest temperature was -3.6°C from the night of March 08 to the morning of March 9, and at noon on March 10, a total of 90 pear blossoms adjacent to each tree crown, middle and bottom were counted , where the flowers turn black or brown is the damaged flower (if 90, the damaged flower ≤ 1 is regarded as not frozen; 2 ≤ damaged flower ≤ 5 is regarded as slightly frozen, 6 ≤ damaged flower < 20 is rega...
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