Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

L-tyrosine detection method based on composite film modified electrode and sensor

A technology for modifying electrodes and detection methods, applied in the field of chemical/biological sensing

Pending Publication Date: 2019-08-02
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no electrochemical sensor based on CuS nanosheet (CuS NS) composite film for the detection of L-Tyr has been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • L-tyrosine detection method based on composite film modified electrode and sensor
  • L-tyrosine detection method based on composite film modified electrode and sensor
  • L-tyrosine detection method based on composite film modified electrode and sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] All the reagents used in the examples are of analytical grade (AR), and the experimental water is all ultrapure water (resistivity ≥ 18.3 MΩ·cm). In the following description, the descriptions of amino acids all use English abbreviations.

[0036] 1. Experimental process

[0037] 1. Preparation of CuS NS-CS / F-MWCNTs composites

[0038] Acidified multi-walled carbon nanotubes (F-MWCNTs) were prepared by blending acidification method to carboxylate the surface. The specific steps are: (1) Weigh 0.3000g multi-walled carbon nanotubes and 30mL H 2 SO 4 / HNO 3 Mixed acid (volume ratio: 3:1; (2) Ultrasonic dispersion at room temperature for 4 hours, then placed in an oil bath at 120°C and stirred for 2 hours; (4) Dilute the mixed solution with ultrapure water, and centrifuge in a high-speed centrifuge after cooling (5) wash with ethanol three times and centrifuge, then place the product in an electric blast drying oven (60°C) and dry it into powder, and store it at room t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
thicknessaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an L-tyrosine detection method based on a composite film modified electrode. The method comprises steps of preparation of CuS NS CS / F MWCNTs composite materials, preparation ofthe CuS NS-CS / F MWCNTs / GCE composite film modified electrode, and L-tyrosine detection and the like. An acidized carbon nano-tube is loaded with a CuS nanosheet (CuS NS), and is modified onto a glassy carbon electrode with chitosan (CS) being as a dispersant to prepare the CuS NS-CS / F MWCNTs / GCE composite film modified electrode. The result shows that the modified electrode has good a good electrocatalytic oxidation characteristic for the L-Tyr, and can be used for measurement of L-Tyr in pig serum samples; lower detection limit reaches 4.9*10<-9>mol / L; and the modified electrode has a potential application value in the field of biological analysis and detection.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing, and in particular relates to a method for detecting L-tyrosine based on a composite membrane modified electrode and a sensor. Background technique [0002] L-tyrosine (L-Tyr), as a physiologically essential amino acid for humans and animals, plays an important role in their metabolism, growth and development. It is also a building block of proteins and a key precursor of melanin and neurotransmitters. It's found in foods like soybeans, fish, eggs, milk, and bananas. However, excessive tyrosine in the human body will cause tyrosinemia, which is a rare autosomal recessive disease caused by abnormal metabolism of L-Tyr. This disease has irreversible damage to the human body, especially It is liver failure, neuralgia, rickets and other diseases of newborns; too low tyrosine content will cause albinism and brown yellow disease. The content of tyrosine will also affect the nutrit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48B82Y5/00B82Y15/00B82Y30/00
CPCG01N27/3278G01N27/3277G01N27/308G01N27/48B82Y30/00B82Y5/00B82Y15/00
Inventor 曹忠朱钦刘楚丰赛飞何军意肖忠良
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com