L-tyrosine detection method based on composite film modified electrode and sensor

A technology for modifying electrodes and detection methods, applied in the field of chemical/biological sensing

Pending Publication Date: 2019-08-02
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no electrochemical sensor based on CuS nanosheet (CuS NS) composite film for the detection of L-Tyr has been reported.

Method used

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  • L-tyrosine detection method based on composite film modified electrode and sensor
  • L-tyrosine detection method based on composite film modified electrode and sensor
  • L-tyrosine detection method based on composite film modified electrode and sensor

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Embodiment Construction

[0035] All the reagents used in the examples are of analytical grade (AR), and the experimental water is all ultrapure water (resistivity ≥ 18.3 MΩ·cm). In the following description, the descriptions of amino acids all use English abbreviations.

[0036] 1. Experimental process

[0037] 1. Preparation of CuS NS-CS / F-MWCNTs composites

[0038] Acidified multi-walled carbon nanotubes (F-MWCNTs) were prepared by blending acidification method to carboxylate the surface. The specific steps are: (1) Weigh 0.3000g multi-walled carbon nanotubes and 30mL H 2 SO 4 / HNO 3 Mixed acid (volume ratio: 3:1; (2) Ultrasonic dispersion at room temperature for 4 hours, then placed in an oil bath at 120°C and stirred for 2 hours; (4) Dilute the mixed solution with ultrapure water, and centrifuge in a high-speed centrifuge after cooling (5) wash with ethanol three times and centrifuge, then place the product in an electric blast drying oven (60°C) and dry it into powder, and store it at room t...

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Abstract

The invention discloses an L-tyrosine detection method based on a composite film modified electrode. The method comprises steps of preparation of CuS NS CS / F MWCNTs composite materials, preparation ofthe CuS NS-CS / F MWCNTs / GCE composite film modified electrode, and L-tyrosine detection and the like. An acidized carbon nano-tube is loaded with a CuS nanosheet (CuS NS), and is modified onto a glassy carbon electrode with chitosan (CS) being as a dispersant to prepare the CuS NS-CS / F MWCNTs / GCE composite film modified electrode. The result shows that the modified electrode has good a good electrocatalytic oxidation characteristic for the L-Tyr, and can be used for measurement of L-Tyr in pig serum samples; lower detection limit reaches 4.9*10<-9>mol / L; and the modified electrode has a potential application value in the field of biological analysis and detection.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing, and in particular relates to a method for detecting L-tyrosine based on a composite membrane modified electrode and a sensor. Background technique [0002] L-tyrosine (L-Tyr), as a physiologically essential amino acid for humans and animals, plays an important role in their metabolism, growth and development. It is also a building block of proteins and a key precursor of melanin and neurotransmitters. It's found in foods like soybeans, fish, eggs, milk, and bananas. However, excessive tyrosine in the human body will cause tyrosinemia, which is a rare autosomal recessive disease caused by abnormal metabolism of L-Tyr. This disease has irreversible damage to the human body, especially It is liver failure, neuralgia, rickets and other diseases of newborns; too low tyrosine content will cause albinism and brown yellow disease. The content of tyrosine will also affect the nutrit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48B82Y5/00B82Y15/00B82Y30/00
CPCB82Y5/00B82Y15/00B82Y30/00G01N27/308G01N27/3277G01N27/3278G01N27/48
Inventor 曹忠朱钦刘楚丰赛飞何军意肖忠良
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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