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L-glutamic acid detection method and sensor based on polymer film modified electrode

A technology for modifying electrodes and detection methods, applied in the field of chemical/biological sensing

Pending Publication Date: 2019-09-10
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, no enzyme-modified electrodes based on L-glutamic acid based on polytryptophan (PTrp) loaded carbon nanotubes have been reported.

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  • L-glutamic acid detection method and sensor based on polymer film modified electrode

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Embodiment Construction

[0031] All the reagents used in the examples are of analytical grade (AR), and the experimental water is all ultrapure water (resistivity ≥ 18.3 MΩ·cm). In the following description, the descriptions of amino acids all use English abbreviations.

[0032] 1. Experimental process

[0033] 1. Preparation of MWCNTs

[0034] Put 200g MWCNTs in the mixed acid (40mL) of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1 and mix them, then stir and reflux in an oil bath at 150°C and heat for 15min. After centrifugation for 5 min, the supernatant was discarded, and the lower precipitate was the acidified MWCNTs. The product was washed three times with ultrapure water to remove excess acid, and then dried overnight at 75°C in a vacuum oven. Finally, the The product is ground and pulverized for later use.

[0035] 2. Preparation of PTrp / MWCNTs / GCE polymer membrane modified electrode

[0036] The surface of the glassy carbon electrode (GCE, 3 mm in diam...

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Abstract

The invention discloses anL-glutamic acid (L-Glu) detection method and sensor based on a polymer film modified electrode.The L-glutamic acid (L-Glu) detection method comprises the steps of preparing MWCNTs, preparing a PTrp / MWCNTs / GCE electrode, detecting L-glutamic acidand the like. The results show that in an acetic acid-sodium acetate buffer solution, adifferential pulse volt-ampere response peak current of the modified electrode and the concentration of L-Glu areina goodlinearrelationshipwithin the range of 5.000*10<-8>-1.500*10<-5> mol / L, and the detection limit is 2.580*10<-8> mol / L (S / N=3). The electrode is high in selectivity and good in reproducibility and stability. The electrode is used for the determination of L-Glu in pig serum samples, and the detection results are consistentwith thedetection results of a high performance liquid chromatography, the recovery rate reaches 94.8%-104.5%, it is indicated that the electrode is expected to be a new approach of enzyme-free detection of glutamic acid, and the L-glutamic acid (L-Glu) detection method and sensor can be applied tothe fields of life analysis and animal nutrition.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing, and in particular relates to a method for detecting L-glutamic acid based on a polymer film modified electrode and a sensor. Background technique [0002] L-glutamic acid (L-Glu) is one of the 20 common amino acids in living organisms. As a flavor additive, it widely exists in food and plays a very important role in the fields of life and health, clinical medicine, and food processing. . L-glutamic acid is an important excitatory neurotransmitter in mammals. It is related to certain behavioral patterns such as learning and memory. Changes in the concentration of L-glutamic acid in a specific area of ​​the brain are associated with Alzheimer's are closely related to diseases such as Parkinson's disease. Moreover, due to the lack of L-glutamic acid in foods such as vegetables, etc., L-glutamic acid is usually added to diet, food and drug formulations, however excessive consump...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/48B82Y15/00
CPCG01N27/3277G01N27/308G01N27/48B82Y15/00
Inventor 曹忠唐飞何军意段薇邹浩云肖忠良
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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