L-glutamic acid detection method and sensor based on polymer film modified electrode

A technology for modifying electrodes and detection methods, applied in the field of chemical/biological sensing

Pending Publication Date: 2019-09-10
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no enzyme-modified electrodes based on L-glutamic acid ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • L-glutamic acid detection method and sensor based on polymer film modified electrode
  • L-glutamic acid detection method and sensor based on polymer film modified electrode
  • L-glutamic acid detection method and sensor based on polymer film modified electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] All the reagents used in the examples are of analytical grade (AR), and the experimental water is all ultrapure water (resistivity ≥ 18.3 MΩ·cm). In the following description, the descriptions of amino acids all use English abbreviations.

[0032] 1. Experimental process

[0033] 1. Preparation of MWCNTs

[0034] Put 200g MWCNTs in the mixed acid (40mL) of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1 and mix them, then stir and reflux in an oil bath at 150°C and heat for 15min. After centrifugation for 5 min, the supernatant was discarded, and the lower precipitate was the acidified MWCNTs. The product was washed three times with ultrapure water to remove excess acid, and then dried overnight at 75°C in a vacuum oven. Finally, the The product is ground and pulverized for later use.

[0035] 2. Preparation of PTrp / MWCNTs / GCE polymer membrane modified electrode

[0036] The surface of the glassy carbon electrode (GCE, 3 mm in diam...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses anL-glutamic acid (L-Glu) detection method and sensor based on a polymer film modified electrode.The L-glutamic acid (L-Glu) detection method comprises the steps of preparing MWCNTs, preparing a PTrp/MWCNTs/GCE electrode, detecting L-glutamic acidand the like. The results show that in an acetic acid-sodium acetate buffer solution, adifferential pulse volt-ampere response peak current of the modified electrode and the concentration of L-Glu areina goodlinearrelationshipwithin the range of 5.000*10<-8>-1.500*10<-5> mol/L, and the detection limit is 2.580*10<-8> mol/L (S/N=3). The electrode is high in selectivity and good in reproducibility and stability. The electrode is used for the determination of L-Glu in pig serum samples, and the detection results are consistentwith thedetection results of a high performance liquid chromatography, the recovery rate reaches 94.8%-104.5%, it is indicated that the electrode is expected to be a new approach of enzyme-free detection of glutamic acid, and the L-glutamic acid (L-Glu) detection method and sensor can be applied tothe fields of life analysis and animal nutrition.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing, and in particular relates to a method for detecting L-glutamic acid based on a polymer film modified electrode and a sensor. Background technique [0002] L-glutamic acid (L-Glu) is one of the 20 common amino acids in living organisms. As a flavor additive, it widely exists in food and plays a very important role in the fields of life and health, clinical medicine, and food processing. . L-glutamic acid is an important excitatory neurotransmitter in mammals. It is related to certain behavioral patterns such as learning and memory. Changes in the concentration of L-glutamic acid in a specific area of ​​the brain are associated with Alzheimer's are closely related to diseases such as Parkinson's disease. Moreover, due to the lack of L-glutamic acid in foods such as vegetables, etc., L-glutamic acid is usually added to diet, food and drug formulations, however excessive consump...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/327G01N27/30G01N27/48B82Y15/00
CPCB82Y15/00G01N27/308G01N27/3277G01N27/48
Inventor 曹忠唐飞何军意段薇邹浩云肖忠良
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap