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Application of Hydroxyl-terminated Dodecanoic Acid in Fruit and Vegetable Preservation

A technology of hydroxy-terminated dodecanoic acid and gene, which is applied in the field of application of hydroxy-terminated dodecanoic acid in the preservation of fruits and vegetables, and can solve the problems that the application of hydroxy-terminated dodecanoic acid has not been reported.

Active Publication Date: 2022-06-28
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, the application of terminal hydroxydodecanoic acid in the field of fruit and vegetable preservation has not been reported

Method used

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  • Application of Hydroxyl-terminated Dodecanoic Acid in Fruit and Vegetable Preservation
  • Application of Hydroxyl-terminated Dodecanoic Acid in Fruit and Vegetable Preservation
  • Application of Hydroxyl-terminated Dodecanoic Acid in Fruit and Vegetable Preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Reconstituted hydroxy-terminated dodecanoic acid with DMSO to 500 mg mL -1 mother liquor, diluted with water to 500mg L -1 , spray evenly on the surface of freshly harvested tomato fruits at green ripening stage, and spray only once. The spraying is based on the fact that the surface of the fruit is evenly covered with mist-like water droplets as the standard, and sterile water is used as the control. The samples were photographed for 8 consecutive days to observe changes in appearance, and samples were taken every 2 days to determine the lycopene content. The samples on the 4th day of treatment were taken to determine the expression levels of lycopene synthesis-related genes (SlPDS1, SlPSY1, and SlCRTISO). like figure 1 As shown, compared with the control, the above results show that hydroxy-terminated dodecanoic acid can effectively delay the color change of tomato fruit.

Embodiment 2

[0032] Taking sterile water as a control, the hydroxy-terminated dodecanoic acid (500 mg L -1 ) sprayed evenly on the surface of the freshly harvested tomato fruit, only once, and sprayed on the surface evenly covered with mist-like water droplets as the standard. On the second day of treatment, the effect of chemical treatment on the ethylene release of tomato fruit was determined. Take the samples on the 4th day of treatment, and measure the expression levels of genes related to ethylene synthesis (SlACO1, SlACO3, SlACS2, SlACS4) and signal transduction (SlCTR2, SlEIL1, SlEIL3, SlERF2, SlETR1, SlETR4). The results are as follows figure 2 shown. Compared with the control, the above results indicated that terminal hydroxydodecanoic acid can significantly reduce the ethylene release of tomato fruit and down-regulate the expression levels of related genes.

Embodiment 3

[0034] Reconstituted hydroxy-terminated dodecanoic acid with DMSO to 500 mg mL -1 mother liquor, diluted with water to 500mg L -1 Spray evenly on the surface of freshly harvested tomato fruits at the green maturity stage, and only spray once. The spraying is based on the fact that the surface of the fruit is evenly covered with mist-like water droplets, and sterile water is used as the control. The samples were photographed for 8 consecutive days to observe changes in appearance, and samples were taken every 2 days to determine the changes in the enzymatic activities of polygalacturonase and pectin methylesterase. Samples were taken on the 4th day of treatment to determine the expression levels of genes related to cell wall structure. like image 3 Compared with the control, the above results show that terminal hydroxydodecanoic acid can effectively reduce the enzymatic activities of polygalacturonase and pectin methylesterase, and significantly down-regulate cell wall struc...

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Abstract

The invention belongs to the technical field of fresh-keeping of fruits and vegetables, and in particular relates to the application of hydroxy-terminated dodecanoic acid in fresh-keeping of fruits and vegetables. The study found that exogenous spraying of terminal hydroxydodecanoic acid on the surface of tomato fruit can significantly delay the ripening process of tomato fruit, and applying it to Botrytis cinerea or the surface of fruit can significantly reduce the pathogenicity of pathogenic bacteria and control the occurrence of postharvest diseases of fruits and vegetables. But so far, the application of this substance in the field of fruit and vegetable preservation has not been reported.

Description

technical field [0001] The invention belongs to the technical field of fresh-keeping of fruits and vegetables, in particular to the application of hydroxy-terminated dodecanoic acid in fresh-keeping of fruits and vegetables. Background technique [0002] Tomato fruit is rich in nutrients and delicious in taste, but tomato fruit is a climacteric fruit, which matures quickly after harvest, has a short shelf life, and is extremely intolerant of storage. In addition, fresh fruits and vegetables are rich in nutrients and high in cellular water content. After harvesting, they are easily rotten and deteriorated due to microbial infection, losing their commercial value and causing huge losses. Therefore, delaying the post-harvest ripening process of fruits and controlling the occurrence of post-harvest diseases of fruits and vegetables is of great significance for the preservation of fruits and vegetables. [0003] The use of hydroxy-terminated dodecanoic acid has not been reported...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B7/154A23B7/16
CPCA23B7/154A23B7/16A23V2002/00A23V2200/10
Inventor 田世平陈彤李博强张占全秦国政徐勇
Owner INST OF BOTANY CHINESE ACAD OF SCI