Rapid detection method of mycobacterium marinum nucleic acid and kit thereof

A technology of mycobacterium and detection method, which is applied in the field of detection method of Mycobacterium marinum nucleic acid and its kit, which can solve the problems of delaying the diagnosis and treatment time of patients

Pending Publication Date: 2019-08-16
USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Mycobacterium marinum is a fast-growing mycobacterium, it still takes at least one to two weeks to isolate and cultu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid detection method of mycobacterium marinum nucleic acid and kit thereof
  • Rapid detection method of mycobacterium marinum nucleic acid and kit thereof
  • Rapid detection method of mycobacterium marinum nucleic acid and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0098] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0099] The operation process of a rapid detection method of Mycobacterium marinum nucleic acid is as follows:

[0100] 1. Collect the purulent secretions of suspected Mycobacterium marinum infection patients.

[0101] 2. Use the QIAamp DNA Mini Kit from QIAGEN to extract the DNA nucleic acid in the secretion. It should be noted that kits from other companies are also available, and this is just an example.

[0102] 3. Design the following 5 primers and probes, and submit them to Sangon Bioengineering (Shanghai) Co., Ltd. for synthesis:

[0103] SEQ ID NO.1: 5'-TGT ACC TGA AGG AGC GCA GT

[0104] SEQ ID NO.2: 5'-Biotin-GGG CTC CTT TGT TGA GGT TG

[0105] SEQ ID NO.3: 5'-6-FAM-GTG TCG GAT CGG GCC ACG TC

[0106] SEQ ID NO.4: 5'-GGG CTC CTT TGT TGA GGT TGG ACC AGC CGC GCG ATA GCA A

[0107] SEQ ID NO.5: 5'-TTG GTT GAC ACT CGA GGC AC

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a rapid detection method of mycobacterium marinum nucleic acid and a kit thereof. The detection method comprises the following steps: collecting purulent secretion of a suspected mycobacterium marinum infected person; extracting DNA nucleic acid in secretion; designing primers and probes; preparing a mycobacterium marinum nucleic acid detection reagent; taking DNA nucleic acid of focus secretion as an amplification template, and carrying out constant-temperature amplification; and after the constant-temperature amplification is finished, qualitatively judging a result by using a disposable nucleic acid detection device. According to the rapid detection method, mycobacteria do not need to be separated and cultured, and the mycobacterium marinum nucleic acid is directly detected from focus secretion, and the method has the advantages of short time consumption and simplicity and convenience in operation. By the primers and probes which are designed by a unique method, detection sensitivity is high, and the specificity is good. The preferable and optimal working concentration range of the primers and probes is selected through experiments, so that the sensitivity and specificity of detection of the kit are further improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for detecting mycobacterium marinum nucleic acid and a kit thereof. Background technique [0002] Mycobacterium marinum is a non-tuberculous mycobacterium that grows in freshwater and oceans. It mainly infects aquatic organisms such as fish, frogs, and shrimps. It can also infect humans through tiny wounds on the skin surface, forming granuloma-like lesions . Most clinical patients are infected by hand stabbing by aquatic products or swimming in polluted waters. The optimum growth temperature of Mycobacterium marinum is about 32°C, and the bacterial growth is inhibited when the temperature rises to 37°C. Therefore, most of the clinically discovered infection foci are located in superficial places with low temperature such as limbs and skin, and patients with low immunity may develop systemic infection. Symptoms of infection generally manifest as protracted skin lum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689Y02A50/30
Inventor 李晓非尤其敏胡林刘红伟张建瑞欧阳兵吕松琴黄山李松鹏张瑜书梁桂亮
Owner USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap