Rapid detection method of mycobacterium marinum nucleic acid and kit thereof

Abstract

The invention discloses a rapid detection method of mycobacterium marinum nucleic acid and a kit thereof. The detection method comprises the following steps: collecting purulent secretion of a suspected mycobacterium marinum infected person; extracting DNA nucleic acid in secretion; designing primers and probes; preparing a mycobacterium marinum nucleic acid detection reagent; taking DNA nucleic acid of focus secretion as an amplification template, and carrying out constant-temperature amplification; and after the constant-temperature amplification is finished, qualitatively judging a result by using a disposable nucleic acid detection device. According to the rapid detection method, mycobacteria do not need to be separated and cultured, and the mycobacterium marinum nucleic acid is directly detected from focus secretion, and the method has the advantages of short time consumption and simplicity and convenience in operation. By the primers and probes which are designed by a unique method, detection sensitivity is high, and the specificity is good. The preferable and optimal working concentration range of the primers and probes is selected through experiments, so that the sensitivity and specificity of detection of the kit are further improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for detecting mycobacterium marinum nucleic acid and a kit thereof. Background technique [0002] Mycobacterium marinum is a non-tuberculous mycobacterium that grows in freshwater and oceans. It mainly infects aquatic organisms such as fish, frogs, and shrimps. It can also infect humans through tiny wounds on the skin surface, forming granuloma-like lesions . Most clinical patients are infected by hand stabbing by aquatic products or swimming in polluted waters. The optimum growth temperature of Mycobacterium marinum is about 32°C, and the bacterial growth is inhibited when the temperature rises to 37°C. Therefore, most of the clinically discovered infection foci are located in superficial places with low temperature such as limbs and skin, and patients with low immunity may develop systemic infection. Symptoms of infection generally manifest as protracted skin lum...

AI Technical Summary

Problems solved by technology

Although Mycobacterium marinum is a fast-growing mycobacterium, it still takes at least one to two weeks to isolate and culture, and the time-consuming follow-up identification of the strains greatly delays the diagnosis and treatment time of patients

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