Flow cytometer optical system
A flow cytometer and optical system technology, applied in the field of cell detection and analysis, can solve the problems of limited space and the inability to place multiple photoelectric sensors and spectroscopic devices, so as to enhance stability, overcome the deterioration or failure of measurement results, The effect of increasing the error
Pending Publication Date: 2019-08-20
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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[0004] In view of this, an embodiment of the present invention provides a flow cytometer optical system to solve the problem that the existing flow cytometer composed of multiple excitation lights is limited in space and cannot place multiple photoelectric sensors and spectroscopic devices. technical issues
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[0023] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0024] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, ...
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The invention discloses a flow cytometer optical system. A channel for enabling a to-be-detected substance to pass through is arranged in a flow chamber; an excitation light source of a light source module forms non-coincident focusing spots in the channel; a light collecting module is used for collecting scattered light and / or fluorescence, converging the light and then outputting the light; a forward detection module is configured to receive and detect the scattered light; a fluorescence detection module includes an optical signal transmission module, a light splitting module and a detectionmodule; the optical signal transmission module is arranged at any position that is not intersected with each other in a full aperture range of an optical lens for deflecting the optical signals outputted by the light collecting module; the light splitting module is used for outputting the deflected optical signals; and the detection module is used for detection the optical signals. Thus, througharranging multiple photoelectric sensors and the light splitting device in the fluorescence detection module, the problems that a measurement result is degraded or invalid due to slight movement of the light collecting module when an optical fiber is adopted for transmission can be solved, and the stability of the system is enhanced.
Description
technical field [0001] The invention relates to the technical field of cell detection and analysis, in particular to an optical system of a flow cytometer. Background technique [0002] Flow cytometry is an instrument that can quickly analyze the characteristics (such as size, refractive index, complexity of internal structure, etc.) of cells or some particles (such as polystyrene microspheres). The sample containing cells is compressed and focused by the sheath fluid, and forms a laminar flow after entering the fluid pool. The cells are compressed on the sample streamline and pass through the laser spot one by one. The detector is tested in front of the optical axis and lateral to the optical axis. The scattered light generated when the cells pass through the laser spot, and the specific fluorescence generated by the fluorescent dyes carried by them, can test the biophysical and biochemical characteristics of cells or tiny particles. [0003] With the innovation of technol...
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IPC IPC(8): G01N15/14
CPCG01N15/1436
Inventor 陈忠祥王策吴云良裴智果钟金凤马玉婷严心涛宋飞飞
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI