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Method for improving detection sensitivity of carbon nanotube labeled test strip biosensor

A biosensor and detection sensitivity technology, which is applied in the direction of biological testing, instruments, measuring devices, etc., can solve the problem of insufficient detection sensitivity of biosensors, and achieve the effects of fast detection means, good signal effect, good reproducibility and specificity

Pending Publication Date: 2019-09-13
ANHUI SCI & TECH UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for improving the detection sensitivity of carbon nanotube-labeled test strip biosensors for the problem that the detection sensitivity of existing carbon nanotube-labeled test strip biosensors is not high enough

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Embodiment Construction

[0030] In order to describe in detail the technical content, achieved objectives and effects of the present invention, the following will be described in conjunction with the embodiments.

[0031] The most critical idea of ​​the present invention is: use carboxyl-modified multi-walled carbon nanotubes as markers to prepare test strip biosensors as raw materials, and use the double-antibody sandwich method to detect the target protein. After the adjustment, the influence on the detection results was analyzed separately, and multiple conditions that had a greater impact on the detection sensitivity of the test strip biosensor were obtained, and then multiple sets of data were set to compare the corresponding response signals, and the corresponding response signal was selected when the response signal was the largest. Conditions to get the optimal conditions;

[0032]Under the optimal experimental conditions, the response signal has a good linear relationship with rabbit IgG in t...

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Abstract

The invention belongs to the technical field of detection of immunodetection sensors, and particularly relates to a method for improving the detection sensitivity of a carbon nanotube labeled test strip biosensor. A labeled antibody compound is prepared from a carboxyl-modified multiwalled carbon nanotube as a raw material; the conditions in the processes of preparing and detecting the test stripbiosensor are sequentially adjusted; when the concentration of rabbit IgG is 5ng / ml, corresponding signals for a detection line under the change of the conditions are obtained, and analysis is carriedout; and the main conditions influencing the detection sensitivity of the carbon nanotube labeled test strip biosensor are obtained, which include the addition amount of an antibody when the labeledantibody compound is prepared, the concentration of the goat anti-rabbit IgG on the detection line, the amount of the labeled antibody compound on a binding pad and the concentration of BSA in a detection buffer solution. Compared with the prior art, the method has the following advantages: under the optimal conditions, the detection limit is 0.1ng / ml; and the method can be used for simply and rapidly carrying out super-sensitive detection on protein.

Description

technical field [0001] The invention belongs to the detection technical field of immunodetection sensors, and in particular relates to a method for improving the detection sensitivity of a carbon nanotube-labeled test strip biosensor. Background technique [0002] Lateral flow immunoassay, a paper-based biosensor for real-time monitoring, has attracted much attention due to its low cost and simple operation. Another major advantage of lateral flow immunoassay is that it can be performed on a variety of biological samples, including plasma, sweat, saliva, serum, urine, and whole blood. In addition, the sample size required for testing is much smaller than that required in routine testing. Therefore, lateral flow immunoassay, as a very practical and portable analytical tool, has been widely used in the rapid detection of proteins. Currently, various nanoparticles including gold nanoparticles, quantum dots, magnetic nanoparticles, upconversion luminescent nanoparticles, and a...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558G01N33/532
CPCG01N33/6854G01N33/558G01N33/532
Inventor 刘国东范川邱万伟钱立生李坤
Owner ANHUI SCI & TECH UNIV
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