Application of cephalosporin antibiotics as preparation of anticancer drugs and anticancer drugs
A technology of anticancer drugs and antibiotics, which is applied in the field of drug research and can solve problems such as hypersensitivity of cancer patients
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Embodiment 1
[0061] Example 1: Cephalosporin antibiotics (except ceftriaxone) have significant and cell-targeted anticancer effects in vitro.
[0062] In vitro anticancer test method: cephalosporin antibiotics were dissolved in sterile normal saline, and the in vitro anticancer test was carried out according to the final concentration of 25, 50, 100, 200, 400 μg / ml. Human cancer cells in the logarithmic growth phase were collected, suspended in DMEM / F12 complete culture medium without antibiotics supplemented with 10% fetal bovine serum (FBS), and inoculated on a 96-well plate, and 100 µl of cell suspension was added to each well (except for MCF-7 Inoculate 1×10 per well 4 1 cells, the rest of the cancer cell lines were inoculated with 5×10 cells per well. 3 cells). 37°C, 5% CO 2 After culturing overnight in the incubator, suck out the culture medium, and then add 200 μl of culture medium containing corresponding concentrations of cephalosporin antibiotics according to the design (the c...
Embodiment 2
[0066] Example 2: Cephalosporin antibiotics (except ceftriaxone) have selective anticancer effects in vitro
[0067] The experimental method is the same as the in vitro anti-cancer experiment in Example 1, using human normal vascular endothelial cells ECV-304 to compare the selective cytotoxicity. In vitro cell culture experiments found that under the same dose and test conditions, cephalosporin antibiotics had almost no cytotoxic effect on human normal vascular endothelial cell line ECV-304, or the cytotoxic effect was significantly lower than that on many human cancer cell lines. Cytotoxicity. The inhibitory effect of cephalosporin antibiotics (except ceftriaxone) on nasopharyngeal carcinoma CNE2 and normal cells in vitro is very significantly different, see image 3 .
Embodiment 3
[0068] Example 3: In vivo anticancer effect of cefotaxime sodium on nasopharyngeal carcinoma
[0069] Anti-transplant human nasopharyngeal carcinoma test method in nude mice: prepare human nasopharyngeal carcinoma cell samples by cell culture, adjust the cell concentration to 4×10 with sterile, FBS-free DMEM / F12 culture medium 7 cells / ml to obtain a cell suspension. For balb / c nude mice (body weight 19-21g) that had been adaptively fed for 3 days, each mouse was subcutaneously inoculated with 0.1ml of cell suspension on the right back. Nude mice were bred in SPF laboratory. Measure the long diameter (a) and transverse diameter (b) of each tumor-bearing mouse tumor with a vernier caliper with automatic reading, using the formula: V=a×b 2 / 2 to calculate the tumor volume per mouse. On the 8th day after cell inoculation, most mice had subcutaneous tumors visible to the naked eye, and the average tumor volume reached 200-250mm 3 Left and right, the tumor-bearing mice were stra...
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