Application of cephalosporin antibiotics as preparation of anticancer drugs and anticancer drugs

A technology of anticancer drugs and antibiotics, which is applied in the field of drug research and can solve problems such as hypersensitivity of cancer patients

Active Publication Date: 2021-12-28
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] "Auxiliary anti-cancer health products" refer to health products used for the adjuvant treatment of cancer patients during the treatment of cancer patients, which may have a sensitizing effect on the treatment of cancer patients, but not for the purpose of treatment

Method used

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  • Application of cephalosporin antibiotics as preparation of anticancer drugs and anticancer drugs
  • Application of cephalosporin antibiotics as preparation of anticancer drugs and anticancer drugs
  • Application of cephalosporin antibiotics as preparation of anticancer drugs and anticancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Cephalosporin antibiotics (except ceftriaxone) have significant and cell-targeted anticancer effects in vitro.

[0062] In vitro anticancer test method: cephalosporin antibiotics were dissolved in sterile normal saline, and the in vitro anticancer test was carried out according to the final concentration of 25, 50, 100, 200, 400 μg / ml. Human cancer cells in the logarithmic growth phase were collected, suspended in DMEM / F12 complete culture medium without antibiotics supplemented with 10% fetal bovine serum (FBS), and inoculated on a 96-well plate, and 100 µl of cell suspension was added to each well (except for MCF-7 Inoculate 1×10 per well 4 1 cells, the rest of the cancer cell lines were inoculated with 5×10 cells per well. 3 cells). 37°C, 5% CO 2 After culturing overnight in the incubator, suck out the culture medium, and then add 200 μl of culture medium containing corresponding concentrations of cephalosporin antibiotics according to the design (the c...

Embodiment 2

[0066] Example 2: Cephalosporin antibiotics (except ceftriaxone) have selective anticancer effects in vitro

[0067] The experimental method is the same as the in vitro anti-cancer experiment in Example 1, using human normal vascular endothelial cells ECV-304 to compare the selective cytotoxicity. In vitro cell culture experiments found that under the same dose and test conditions, cephalosporin antibiotics had almost no cytotoxic effect on human normal vascular endothelial cell line ECV-304, or the cytotoxic effect was significantly lower than that on many human cancer cell lines. Cytotoxicity. The inhibitory effect of cephalosporin antibiotics (except ceftriaxone) on nasopharyngeal carcinoma CNE2 and normal cells in vitro is very significantly different, see image 3 .

Embodiment 3

[0068] Example 3: In vivo anticancer effect of cefotaxime sodium on nasopharyngeal carcinoma

[0069] Anti-transplant human nasopharyngeal carcinoma test method in nude mice: prepare human nasopharyngeal carcinoma cell samples by cell culture, adjust the cell concentration to 4×10 with sterile, FBS-free DMEM / F12 culture medium 7 cells / ml to obtain a cell suspension. For balb / c nude mice (body weight 19-21g) that had been adaptively fed for 3 days, each mouse was subcutaneously inoculated with 0.1ml of cell suspension on the right back. Nude mice were bred in SPF laboratory. Measure the long diameter (a) and transverse diameter (b) of each tumor-bearing mouse tumor with a vernier caliper with automatic reading, using the formula: V=a×b 2 / 2 to calculate the tumor volume per mouse. On the 8th day after cell inoculation, most mice had subcutaneous tumors visible to the naked eye, and the average tumor volume reached 200-250mm 3 Left and right, the tumor-bearing mice were stra...

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Abstract

The invention relates to the application of cephalosporin antibiotics in the preparation of anticancer drugs and the anticancer drugs, belonging to the technical field of drug research. Cephalosporin antibiotics have highly specific anticancer effects on nasopharyngeal carcinoma, and also have obvious anticancer effects on other types of cancer; they are not toxic to normal cells or their toxic effects are much lower than those on cancer cells; they significantly inhibit nasopharyngeal carcinoma in vivo Growth, does not affect animal body weight development; different from the combined effects of clinical anticancer drugs on different cancers. The anticancer activity of cephalosporin antibiotics varies greatly, and ceftriaxone has no anticancer activity. The anticancer activity and selectivity of cephalosporin antibiotics are determined by the side chains of their chemical structures, and the modification of the extranuclear structure is the way to discover and synthesize new anticancer substances. The results showed that cephalosporin antibiotics except ceftriaxone are specific anticancer drugs for nasopharyngeal carcinoma, and can also be used as therapeutic drugs for other cancers, as combined anticancer drugs, or as health products for adjuvant cancer treatment.

Description

technical field [0001] The present invention belongs to the technical field of drug research, and in particular relates to the application of cephalosporin antibiotics (except ceftriaxone) in the preparation of drugs with specific and selective anti-nasopharyngeal cancer effects and the application of other cancer anti-cancer drugs, including The cephalosporin antibiotic has specific and selective anticancer drugs or a pharmaceutical composition with combined anticancer effects. Background technique [0002] Cancer is a major disease that seriously threatens human health and life safety, and is the main cause of death for human beings. According to data from the International Center for Cancer Research, in 2012, the number of malignant tumors in the world reached 14.1 million cases and 8.2 million deaths; among them, the number of nasopharyngeal cancer cases was 86,691, and the number of deaths was 50,831. In 2018, there were 18.1 million new cases of cancer worldwide and 9...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/546A61K31/43A61K31/513A61K31/282A61P35/00A61P35/02C07D501/34C07D501/06A23L33/10
CPCA61K31/546A61K31/43A61K31/513A61K31/282A61P35/00A61P35/02C07D501/34C07D501/06A23L33/10A23V2002/00A61K2300/00A23V2200/308
Inventor 贺小琼
Owner KUNMING MEDICAL UNIVERSITY
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