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Polysaccharide carrier with endocytosis mediation function, and preparation method thereof

A cell and polysaccharide technology, applied in the field of polysaccharide carrier with endocytosis-mediated function and its preparation, can solve the problems of difficult enzymatic degradation, poor affinity, large rejection, etc., and achieve the effect of good transmembrane function

Pending Publication Date: 2019-10-22
湖北亿彤实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, polymer carriers have the problems of poor affinity with organisms, large rejection reactions, and are not easy to be degraded by enzymes in organisms, and the degraded products have relatively large toxic and side effects on organisms. Add extra metabolic burden to the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing a polysaccharide carrier with a cell endocytosis mediating function, specifically comprising the following steps:

[0031] S1. Extract the crude polysaccharide of Inonotus obliquus by alcohol precipitation; after the Inonotus obliquus is dried and pulverized, pass through a 60-mesh sieve to obtain Inonotus obliquus powder, add deionized water to the Inonotus obliquus powder, and place in a constant temperature water bath Heat and reflux in the pot twice, each time for 3 hours, combine the filtrates several times after centrifugation, concentrate with a rotary evaporator, add 4 times the amount of the concentrated solution with an ethanol solution with a volume fraction of 95% for alcohol precipitation, let stand overnight, and freeze after centrifugation Dried crude polysaccharide;

[0032] S2. Use distilled water to make the extracted crude polysaccharide into an aqueous solution. After filtering through a filter membrane, the DEAE-Sepharose CL-6...

Embodiment 2

[0040] A method for preparing a polysaccharide carrier with a cell endocytosis mediating function, specifically comprising the following steps:

[0041]S1. Extract the crude polysaccharide of Inonotus obliquus by alcohol precipitation; after the Inonotus obliquus is dried and pulverized, pass through a 60-mesh sieve to obtain Inonotus obliquus powder, add deionized water to the Inonotus obliquus powder, and place in a constant temperature water bath Heat and reflux in the pot for 4 times, 2 hours each time, combine the filtrates several times after centrifugation, concentrate with a rotary evaporator, add 4 times the amount of the concentrated solution with an ethanol solution with a volume fraction of 95% for alcohol precipitation, let stand overnight, and freeze after centrifugation Dried crude polysaccharide;

[0042] S2. Use distilled water to make the extracted crude polysaccharide into an aqueous solution. After filtering through a filter membrane, the DEAE-Sepharose CL-...

Embodiment 3

[0051] A method for preparing a polysaccharide carrier with a cell endocytosis mediating function, specifically comprising the following steps:

[0052] S1. Extract the crude polysaccharide of Inonotus obliquus by alcohol precipitation; after the Inonotus obliquus is dried and pulverized, pass through a 60-mesh sieve to obtain Inonotus obliquus powder, add deionized water to the Inonotus obliquus powder, and place in a constant temperature water bath Heat and reflux in the pot for 3 times, 3 hours each time, combine the filtrates several times after centrifugation, concentrate with a rotary evaporator, add 4 times the amount of the concentrated solution with an ethanol solution with a volume fraction of 95% for alcohol precipitation, let stand overnight, and freeze after centrifugation Dried crude polysaccharide;

[0053] S2. Use distilled water to make the extracted crude polysaccharide into an aqueous solution. After filtering through a filter membrane, the DEAE-Sepharose CL...

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Abstract

The invention relates to a polysaccharide carrier with an endocytosis mediation function, and a preparation method thereof. A finally prepared Inonotus obliquus polysaccharide iron complex carrier canload Inonotus obliquus polysaccharides well and is capable of binding to an internalization receptor on the surface of a cell at the same time to trigger the activation of the endocytosis function ofthe cell so as to realize transmembrane transport of the polysaccharides, and thus, the Inonotus obliquus polysaccharides can enter the cell well to achieve its function in treating and preventing diseases.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide application, and in particular relates to a polysaccharide carrier with cell endocytosis mediating function and a preparation method thereof. Background technique [0002] Inonotus obliquus belongs to the phylum Fungi, Basidiomycotina, Phytomycetes, Phytomycetes, Inonotus rotaceae, and Inonotus obliquus. At present, the chemical composition of Inonotus obliquus mainly includes lanolin-type steroids Compounds, polysaccharides, polyphenols, lignin, melanin, alkaloids, tannin compounds, folic acid derivatives, and aromatic vanillic acid and syringic acid, etc. Inonotus obliquus is an important species in Russia, Poland, Japan and other countries common folk medicines. At present, relevant researchers have carried out various studies on various active ingredients in Inonotus obliquus, hoping to gradually apply them in the prevention and treatment of malignant tumors, diabetes, cardiovascular d...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K47/36A61K47/22
CPCC08B37/00C08B37/0003A61K47/36A61K47/22Y02A50/30
Inventor 黄仁杰约翰.古斯塔夫.卡尔松杰森.陈
Owner 湖北亿彤实业有限公司
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