Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of solid culture medium for Streptomyces noursii fermentation and its preparation method and application

A technology of Streptomyces noursii and solid medium, applied in the field of microbial fermentation, can solve the problems of less spore production, complex fermentation process, and long fermentation time, and achieve suitable fermentation conditions, stable product quality, and low production cost Effect

Inactive Publication Date: 2021-03-16
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a Streptomyces noursii solid fermentation medium formula and its preparation method and application, to solve the technical problems in the prior art such as complex fermentation process, less spore production by fermentation, long fermentation time and high production cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The invention provides a preparation method for the above-mentioned Streptomyces knowersi fermentation solid medium, comprising the following steps:

[0031] (1) mixing the black soil, soybean dregs powder, rice husk powder, bran, calcium carbonate and water to obtain a mixed material;

[0032] (2) Sterilizing the mixed material to obtain a solid fermentation medium.

[0033] In the invention, the black soil, soybean residue powder, rice husk powder, bran, calcium carbonate and water are firstly mixed to obtain a mixed material. Preferably, in the present invention, the black soil, soybean dregs powder, rice husk powder, bran and calcium carbonate are mixed first, and then water is added to obtain the mixed material.

[0034] After the mixed material is obtained, the present invention sterilizes the mixed material to obtain a solid fermentation medium. In the present invention, the sterilization method is preferably high-pressure steam sterilization, and the temperatu...

Embodiment 1

[0069] A kind of solid medium for Streptomyces knowersi fermentation:

[0070] Raw materials: 30 parts of black soil, 49.5 parts of soybean dregs powder, 10 parts of rice husk powder, 10 parts of bran, 0.5 parts of calcium carbonate and 30 parts of water.

[0071] Preparation method: Weigh soybean dregs powder, rice husk powder, bran, black soil and calcium carbonate according to the proportion of raw materials, mix all raw materials evenly, add water, stir evenly, and then sterilize with high-pressure steam to obtain Streptomyces noersei solid For the fermentation medium, the treatment conditions for high-pressure steam sterilization are as follows: sterilize for 40 minutes at 121° C. and 0.12 MPa pressure.

Embodiment 2

[0073] A kind of solid medium for Streptomyces knowersi fermentation:

[0074] Raw materials: 40 parts of black soil, 32 parts of soybean dregs powder, 15 parts of rice husk powder, 15 parts of bran, 0.5 parts of calcium carbonate and 25 parts of water.

[0075] Preparation method: Weigh soybean dregs powder, rice husk powder, bran, black soil and calcium carbonate according to the proportion of raw materials, mix all raw materials evenly, add water, natural pH, stir evenly, and then undergo high-pressure steam sterilization to obtain Knowles Streptomyces solid fermentation medium, the treatment conditions of high-pressure steam sterilization are as follows: sterilize for 50 minutes under the conditions of 121° C. and 0.15 MPa pressure.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a solid medium for streptomyces noursei fermentation and a preparation method and application of the solid medium, and belongs to the technical field of microbial fermentation.The solid medium for streptomyces noursei fermentation is prepared from the components in parts by weight: 10-50 parts of black soil, 30-60 parts of soybean residue powder, 10-20 parts of rice hull powder, 10-20 parts of bran, 0.5-1 part of calcium carbonate and 25-35 parts of water. The growth amount and sporulation quantity of streptomyces noursei are increased by adopting the solid medium for streptomyces noursei fermentation to ferment and cultivate the streptomyces noursei, and the effective viable bacteria count of spore powder achieves to 4 billion / g to 6 billion / g.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a medium for solid fermentation of Streptomyces noersei and a culture method and application thereof. Background technique [0002] With the strengthening of environmental awareness, people began to gradually reduce the use of chemical pesticides in the prevention and treatment of plant diseases. Biopesticides have the characteristics of less toxic and side effects, safety and good environmental compatibility, and are a new trend in the development of the global pesticide industry. Streptomyces noursei (Streptomyces noursei) is widely distributed in the soil, air and water in nature, and has a strong inhibitory effect on a variety of plant disease pathogens, and can prevent and control diseases including rice blight, soybean root rot, rice stripe leaf blight, etc. . Therefore, Streptomyces noursii has broad application prospects in agricultural biological control...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/57
CPCC12N1/20
Inventor 谷军张小彦于德水
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com