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A strain of Trichoderma aculeatus with growth-promoting effect and its cultivation method and application

A technology of Trichoderma aculeatus and culture medium, which is applied in the field of microorganisms, can solve the problems of reducing the total amount and promoting the growth of crops without any effect, and achieves the effects of increasing ventilation, shortening fermentation period, producing and improving

Active Publication Date: 2021-02-19
KINGENTA ECOLOGICAL ENG GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chlamydospores of Trichoderma aculeatus in this invention obviously promote the volatilization of arsenic in the soil, reduce the total amount of arsenic in the soil, and can effectively repair the soil contaminated by arsenic, but have no effect on the growth promotion of crops

Method used

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  • A strain of Trichoderma aculeatus with growth-promoting effect and its cultivation method and application
  • A strain of Trichoderma aculeatus with growth-promoting effect and its cultivation method and application
  • A strain of Trichoderma aculeatus with growth-promoting effect and its cultivation method and application

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Effect test

Embodiment 1

[0075] A strain of Trichoderma asperellum wood-8, preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on October 21, 2015, address: Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, deposit number: CGMCC NO.11528.

[0076] Preparation of solid fermentation medium

[0077] The preparation method of Trichoderma aculeatus solid fermentation medium comprises the following steps:

[0078] A. Take rice husk, corn stalks, bran, and corn flour according to the above mass ratio, and mix them uniformly to obtain solid materials;

[0079] B. Weigh sucrose, (NH 4 ) 2 SO 4 、KH 2 PO 4 0.5%, MgSO 4 0.25%, FeSO 4 0.003%, mixed together to obtain a mixture of inorganic salts;

[0080] C. Dissolve the inorganic salt mixture in step B in water, mix evenly with the solid material in step A in a mass ratio of 1:1.2, and the pH is natural; the mixed solid medium is steril...

Embodiment 2

[0087] With the Trichoderma asperellum (Trichoderma asperellum) wood-8 bacterial strain culture method described in embodiment 1, difference is:

[0088] (1) Strain activation: the Trichoderma aculeatus strain preserved at 4°C was inoculated on a PDA medium plate, cultivated at 28°C for 4 days, and set aside;

[0089] (2) Prepare the shake flask seed liquid: the Trichoderma aculeatus bacterial classification that will activate is good, extract a ring and be inoculated in the triangular flask that 200ml liquid seed culture medium is housed with the inoculation loop, the triangular flask is placed in the constant temperature oscillation incubator, 28°C, 150rpm, shaking culture for 36h, the obtained shake flask seed solution;

[0090] (3) Preparation of first-class seeds: inoculate the shake flask seed liquid at a ratio of 0.5% to the first-class seed medium, 28° C., 150 rpm, and stir for 36 hours to obtain the first-class seed liquid;

[0091] (4) Solid fermentation: insert the...

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Abstract

The invention relates to a solid fermentation production method of Trichoderma aculeatus. The Trichoderma asperellum strain used in this invention has been preserved in the General Microorganism Center of China General Microorganism Culture Collection Management Committee, the preservation date: October 27, 2015, and the preservation number: CGMCC No.11528. The Trichoderma aculeatus undergoes strain activation, shake flask seed preparation, primary liquid seed cultivation, and then solid fermentation. The solid fermentation medium used in the solid fermentation is composed of rice husk, corn stalk powder, bran or corn flour and inorganic salts. The solid fermentation method for Trichoderma aculeatus of the present invention can produce a large amount of conidia, and the number of spores of Trichoderma aculeatus in the matrix can reach up to 1×10 9 cfu / g~1×10 10 cfu / g, the number of viable fermentation bacteria is high, the production process is pollution-free and does not produce waste, and the production process efficiency is high. The fermented product obtained by fermentation has an obvious growth-promoting effect on crops, and greatly improves the economic benefits of crops.

Description

technical field [0001] The invention relates to a growth-promoting Trichoderma aculeatus and its cultivation method and application, belonging to the technical field of microbes. Background technique [0002] Trichoderma spp. is a filamentous fungus with wide adaptability, against various soil-borne plant pathogenic microorganisms such as Rhizoctonia solani, Pythium spp., and Fusarium spp. It has a good control effect and has a good application prospect in the field of biological control and environmental protection. It is one of the most widely used biocontrol bacteria at present. Trichoderma can control the infection of pathogenic bacteria and the occurrence of diseases through various mechanisms such as antagonism, reparasitism, and secretion of antibiotics. There are many reports at home and abroad on the use of Trichoderma to control plant diseases, especially soil-borne diseases such as root rot, fusarium wilt, sclerotinia and sheath blight, and good control effects h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P21/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 徐文凤顾志光胡红涛范玲超胡兆平
Owner KINGENTA ECOLOGICAL ENG GRP
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