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A kind of Streptomyces roseus flavum solid fermentation medium and its preparation and fermentation method

A technology of Streptomyces roseus flavum and solid fermentation, applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of long fermentation period, less spore production, complex fermentation process, etc., and achieve suitable fermentation conditions , The effect of increasing the amount of spore production and improving production efficiency

Active Publication Date: 2019-10-25
菏泽金正大生态工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, aiming at the deficiencies in the prior art, the object of the present invention is to provide a Streptomyces roseoflavus solid fermentation medium formula, a medium preparation method and a solid fermentation method to solve the problem of complex fermentation process and long fermentation period in the prior art. , low spore production and other technical problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Preparation of Streptomyces roseoflavum solid fermentation medium:

[0050] A In terms of mass percentage, weigh the following materials: 45% of sandy soil, 30.2% of corn flour, 12% of rice husk, 12% of corn stalk powder, and 0.8% of calcium carbonate, and mix them evenly in a metal tray.

[0051] B Add water accounting for 30% of the weight of the solid material, wrap the tray and the material with kraft paper, and apply 1.0Kg / cm at 121°C 3 Sterilize under pressure conditions for 40 minutes to obtain the Streptomyces roseoflavum solid fermentation medium.

[0052] 2. Fermentation method of Streptomyces roseus flavum:

[0053] (1) Shake flask seed liquid preparation: transfer the Streptomyces roseoflavum that has been cultured for 3 days on the solid Gaoshi No. 1 medium to the liquid Gaoshi No. 1 medium; at 25°C, the oscillation frequency of the constant temperature incubator is 150r / min Under the condition of constant temperature shaking culture for 5 days, carry ...

Embodiment 2

[0058] The preparation method of the Streptomyces roseoflavum solid fermentation medium, the fermentation method of Streptomyces roseoflavum are the same as Example 1, and the difference is:

[0059] The proportion of solid raw materials of the Streptomyces roseus yellow solid fermentation medium is different, which is 31.5% of sandy soil, 40% of corn flour, 12% of rice husk, 16% of corn stalk powder, and 0.5% of calcium carbonate.

[0060] During the fermentation process of Streptomyces roseiflavus, step (1) in the shake flask seed liquid preparation step, the selected Streptomyces roseiflavum was cultivated on the solid Gaoshi No. 1 medium for different days, which was 5 days; On Shi No. 1 medium, under the conditions of 28°C and constant temperature incubator oscillation frequency of 170r / min, the constant temperature shaking culture was carried out for 3 days, and the seeds were initially multiplied.

[0061] In the solid fermentation process described in step (3), the moi...

Embodiment 3

[0063] The preparation method of the Streptomyces roseoflavum solid fermentation medium, the fermentation method of Streptomyces roseoflavum are the same as Example 1, and the difference is:

[0064] The raw material ratio of the Streptomyces roseus flavus solid fermentation medium is different, including 30% of sandy soil, 39% of corn flour, 14% of rice husk, 16% of corn stalk powder and 1% of calcium carbonate.

[0065] During the preparation process of the Streptomyces roseoflavum solid fermentation medium, the high-temperature and high-pressure sterilization time of the Streptomyces roseoflavum solid fermentation medium is 30 minutes.

[0066] During the fermentation process of Streptomyces roseiflavum, step (1) in the step of preparing the shake flask seed liquid, transfer Streptomyces roseiflavum to the liquid Gaoshi No. 1 medium, at 26°C, the oscillation frequency of the constant temperature incubator is 160r / min Under the condition of constant temperature and shaking c...

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Abstract

The invention relates to a streptomyces roseoflavus solid fermentation medium and a preparation and fermentation method thereof, and belongs to the field of microorganism fermentation. The medium is prepared from, by weight, solid materials and water, wherein the water accounts for 30% of the solid materials by weight, and the solid materials are prepared from, by weight, 10-49.5% of sandy soil, 30-60% of corn flour, 10-20% of rice hulls, 10-20% of corn stalk powder and 0.5-1% of calcium carbonate. The streptomyces roseoflavus solid fermentation medium prepared through the components is used for liquid-solid dual-phase fermentation, and a streptomyces roseoflavus agent is obtained. The method is simple and easy to implement, and low in cost, the effective viable count of conidial powder reaches 7.8-9.9 billions / g, the production cycle can be effectively shortened, and the production efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a formulation of a Streptomyces roseus flavum solid fermentation medium, preparation of the medium and a solid fermentation method. Background technique [0002] Phytopathogenic nematodes are one of the important pathogens of plant diseases, which cause serious losses to agriculture and forestry every year. With the strengthening of people's awareness of environmental protection, the chemical nematicides that are allowed to be used are gradually withdrawn from the market, and only a few chemical nematicides with low toxicity, high efficiency and low residue are still in use. People are paying more and more attention to microbes to find new solutions to control nematodes. Actinomycete is an important microbial resource for producing antibiotics. Many agricultural antibiotics have been successfully developed to control plant pathogenic nematodes. For example, abamectin produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/465
CPCC12N1/20
Inventor 李新柱胡兆平徐文凤顾志光徐春英
Owner 菏泽金正大生态工程有限公司
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