Biological sterilization preparation and application thereof in controlling bean sprout anthracnose

A technology of biology and preparation, applied in the field of biocidal preparations and its application in the prevention and treatment of bean sprouts anthracnose, which can solve the problems of hidden safety hazards, poisonous bean sprouts, etc., achieve high safety, prevent rot and deterioration, and inhibit the growth of mycelia

Inactive Publication Date: 2019-11-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of the above-mentioned chemicals has curbed the incidence of anthracnose and greatly improved the appearance of bean sprouts, it has also produced some potential safe

Method used

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  • Biological sterilization preparation and application thereof in controlling bean sprout anthracnose
  • Biological sterilization preparation and application thereof in controlling bean sprout anthracnose
  • Biological sterilization preparation and application thereof in controlling bean sprout anthracnose

Examples

Experimental program
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Effect test

Example Embodiment

[0028] Example 1: The effect of deacetylated chitosan with different degrees of deacetylation on the growth of anthracnose pathogen

[0029] The growth rate method was used to determine the effect of deacetylated chitosan with different degrees of deacetylation on the growth of anthracnose mycelium. Among them, the deacetylation degrees of deacetylated chitosan were 54%, 66%, and 88, respectively. %, 90%, 95% and 97%, the test deacetylated chitosan was prepared 1×10 3 mg·L -1 The mass concentration is added to the PDA medium and mixed well to prepare a drug-containing PDA plate containing different deacetylated chitosan. The colony of the bean sprouts anthracnose pathogen strains cultured on the PDA plate is punched with a puncher to get a 5mm diameter plate, and the mycelium is inoculated in the center of the medicated PDA plate with the mycelium face down. The PDA plate without medicine is used as a blank control After placing the above PDA plate in a constant temperature incub...

Example Embodiment

[0037] Example 2: Carboxymethyl chitosan, deacetylated chitosan, sodium benzoate, and a combination of the three and bean sprout anthracnose Influence of bacterial growth

[0038] In this example, carboxymethyl chitosan, deacetylated chitosan (deacetylation degree of 95%), sodium benzoate, carboxymethyl chitosan, deacetylated chitosan (deacetylation degree of 95%) were measured. %) and sodium benzoate in different proportions to prepare the fungicide on the hypha growth and spore germination of Colletotrichum spp., A1 is carboxymethyl chitosan; A2 is deacetylated chitosan ; A3 is sodium benzoate; A4 is carboxymethyl chitosan, deacetylated chitosan and sodium benzoate in a mass ratio of 1:1:1; A5 is carboxymethyl chitosan, deacetylated Chitosan and sodium benzoate are compounded at a mass ratio of 1:5:15; A6 is carboxymethyl chitosan, deacetylated chitosan and sodium benzoate are compounded at a mass ratio of 1:8:20. A7 is a compound of carboxymethyl chitosan, deacetylated chit...

Example Embodiment

[0046] Example 3: The effect of different fungicides on the growth of anthracnose hyphae

[0047] The growth rate method was used to determine the effect of different bactericidal agents on the growth of anthracnose mycelium. The test bactericidal preparations were formulated into 1×10 3 mg·L -1 , 1×10 2 mg·L -1 , 10mg·L -1 , 1mg·L -1 , 0.1mg·L -1 The mass concentration is added to the PDA medium and mixed well to make a drug-containing plate. The colony of the tested strains cultured on the PDA plate was punched with a puncher to get a 5mm diameter plate, and the mycelium was inoculated in the center of the medicated PDA plate with the mycelium face down. The PDA plate without medicine was used as a blank control. The cross method was used to measure the colony diameter of the tested strains and record the data. Comparing with the control, calculate the growth inhibition rate of each fungicide treatment on the pathogen, the inhibition rate (%)=(treatment group colony diameter-...

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Abstract

The invention belongs to the technical field of vegetable sterilization, and discloses a specific biological sterilization preparation DY-6 for bean sprout anthracnose. The biological sterilization preparation DY-6 is prepared from carboxymethyl chitosan, deacetylated chitosan and sodium benzoate, wherein the mass ratio of carboxymethyl chitosan to chitosan to sodium benzoate is (1 to 5) to (5 to12) to (15 to 24). The biological sterilization preparation can significantly inhibit mycelial growth and spore germination of the bean sprout anthracnose, is suitable for controlling the bean sproutanthracnose in the bean sprout production, and has the advantages that the safety is high and no pesticide residues is generated compared with sterilizing agents such as carbendazim and chlorothalonilcommonly used in the market, and the food safety and good quality of bean sprouts are ensured.

Description

technical field [0001] The invention belongs to the technical field of vegetable sterilization, and in particular relates to a biological bactericidal preparation and its application in preventing and treating bean sprout anthracnose. Background technique [0002] Bean sprouts, also known as bean sprouts, are cultivated from the seeds of various cereals, beans or trees under suitable water, temperature, air and dark environmental conditions. Roots or unexpanded cotyledons) for food, including soybean sprouts, mung bean sprouts, black bean sprouts, pea sprouts, broad bean sprouts, etc. The traditional bean sprouts production process adopts technical means such as temperature control and water spraying to produce products with thick hypocotyls, crisp and tender, and short radicles. With the improvement of living standards, people have higher requirements for the palatability of bean sprouts. Bean sprouts that are thick, white and tender, sweet and crisp, and without radicle a...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01N37/10A01P3/00A01G7/06A01G31/00A23B7/08
CPCA01G7/06A01G31/00A01N37/10A01N43/16A23B7/08A23V2002/00A23V2200/10A23V2250/511
Inventor 王汉荣谢昀烨方丽王连平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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