Preparation method of eye drops

Abstract

The invention relates to the field of pharmaceuticals and particularly relates to a preparation method of eye drops. The preparation method comprises the following step: soaking raw material pedicularis verticillata in a sodium chloride isotonic solution for treatment, wherein when the osmotic pressure of the sodium chloride solution is equal to that of cells of pedicularis verticillata, the osmotic pressures inside and outside the cells are balanced, matter exchange is avoided, and component loss is avoided; the active components are fully extracted, a phenylpropanoid glycoside compound is extracted therefrom, so that on one hand, the eye drops have relatively strong antibacterial and anti-inflammatory activities, achieve the effect of transcriptional activation of tissue inflammation genes, and are capable of alleviating inflammation of eyes; on the other hand, the eye drops also contain antioxidant active matters for alleviating fatigue, so that muscle contraction caused by fatiguecan be alleviated, and the eye drops play roles of preventing fatigue and protecting eyeballs.

Description

technical field

[0001] The invention relates to the field of pharmacy, in particular to a preparation method of eye drops. Background technique

[0002] Nowadays, more and more young people suffer from visual fatigue due to long-term use of computers, watching TV, reading books, working in an air-conditioned environment, and studying. They always feel dry eyes, soreness, pain, blurred vision, and even vision loss , so how to protect the eyes and avoid visual fatigue when the working and learning environment cannot be changed? Experts suggest that you should use eye drops specially used to relieve visual fatigue. in human physiological tears. Make the eye drops of various effects also be subjected to the welcome of consumers in general. And the quality problem of the water in the eye drop is also related to the curative effect of the eye drop.

[0003] Eye infection is a common ophthalmic disease. Clinical bacterial research results show that local infection is often cause...

Examples

preparation example Construction

[0021] A preparation method of eye drops, comprising the steps of:

[0022] (1) Take Artemisia annua and add 15% sodium chloride solution at a mass ratio of 1:10, soak it at 20-25°C for 35-45 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and press the mass ratio Add methanol solution with a mass fraction of 50% at 1:8, keep it for 1~2h, ultrasonicate at 250W for 40~60min, centrifuge, take the supernatant and filter it through a 0.45μm microfiltration membrane, take the filtrate to rotary evaporate, and concentrate under reduced pressure to obtain concentrated thing, spare;

[0023] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 400-500 parts of homogenate, 100-120 parts of distilled water, and 2-3 parts by mass. Stir and mix 30% hydrochloric acid solution and 4~5 parts of calcium chloride for 1.5~2 hours, filter, take the fil...

Embodiment 1

[0027] A preparation method of eye drops, comprising the steps of:

[0028] (1) Take Artemisia chinensis and add 15% sodium chloride solution at a mass ratio of 1:10, soak at 20°C for 35 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and add it at a mass ratio of 1:8 The methanol solution with a mass fraction of 50% was kept for 1 h, ultrasonicated at 250W for 40 min, centrifuged, the supernatant was filtered through a 0.45 μm microfiltration membrane, the filtrate was rotary evaporated, concentrated under reduced pressure to obtain a concentrate, and set aside;

[0029] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 400 parts of homogenate, 100 parts of distilled water, 2 parts of 30% hydrochloric acid solution, Stir and mix 4 parts of calcium chloride for 1.5 hours, filter, take the filtrate and add chitosan at a mass ratio o...

Embodiment 2

[0033] A preparation method of eye drops, comprising the steps of:

[0034] (1) Take Artemisia annua and add 15% sodium chloride solution at a mass ratio of 1:10, soak it at 22°C for 40 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and add it at a mass ratio of 1:8 The methanol solution with a mass fraction of 50% was kept for 1~2h, ultrasonicated at 250W for 50min, centrifuged, the supernatant was filtered through a 0.45μm microfiltration membrane, the filtrate was rotary evaporated, concentrated under reduced pressure to obtain a concentrate, and set aside;

[0035] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 450 parts of homogenate, 110 parts of distilled water, 2 parts of 30% hydrochloric acid solution, Stir and mix 4 parts of calcium chloride for 1.7 hours, filter, take the filtrate and add chitosan at a mass ratio of ...

technical field

[0001] The invention relates to the field of pharmacy, in particular to a preparation method of eye drops. Background technique

[0002] Nowadays, more and more young people suffer from visual fatigue due to long-term use of computers, watching TV, reading books, working in an air-conditioned environment, and studying. They always feel dry eyes, soreness, pain, blurred vision, and even vision loss , so how to protect the eyes and avoid visual fatigue when the working and learning environment cannot be changed? Experts suggest that you should use eye drops specially used to relieve visual fatigue. in human physiological tears. Make the eye drops of various effects also be subjected to the welcome of consumers in general. And the quality problem of the water in the eye drop is also related to the curative effect of the eye drop.

[0003] Eye infection is a common ophthalmic disease. Clinical bacterial research results show that local infection is often cause...

Examples

preparation example Construction

[0021] A preparation method of eye drops, comprising the steps of:

[0022] (1) Take Artemisia annua and add 15% sodium chloride solution at a mass ratio of 1:10, soak it at 20-25°C for 35-45 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and press the mass ratio Add methanol solution with a mass fraction of 50% at 1:8, keep it for 1~2h, ultrasonicate at 250W for 40~60min, centrifuge, take the supernatant and filter it through a 0.45μm microfiltration membrane, take the filtrate to rotary evaporate, and concentrate under reduced pressure to obtain concentrated thing, spare;

[0023] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 400-500 parts of homogenate, 100-120 parts of distilled water, and 2-3 parts by mass. Stir and mix 30% hydrochloric acid solution and 4~5 parts of calcium chloride for 1.5~2 hours, filter, take the fil...

Embodiment 1

[0027] A preparation method of eye drops, comprising the steps of:

[0028] (1) Take Artemisia chinensis and add 15% sodium chloride solution at a mass ratio of 1:10, soak at 20°C for 35 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and add it at a mass ratio of 1:8 The methanol solution with a mass fraction of 50% was kept for 1 h, ultrasonicated at 250W for 40 min, centrifuged, the supernatant was filtered through a 0.45 μm microfiltration membrane, the filtrate was rotary evaporated, concentrated under reduced pressure to obtain a concentrate, and set aside;

[0029] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 400 parts of homogenate, 100 parts of distilled water, 2 parts of 30% hydrochloric acid solution, Stir and mix 4 parts of calcium chloride for 1.5 hours, filter, take the filtrate and add chitosan at a mass ratio o...

Embodiment 2

[0033] A preparation method of eye drops, comprising the steps of:

[0034] (1) Take Artemisia annua and add 15% sodium chloride solution at a mass ratio of 1:10, soak it at 22°C for 40 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and add it at a mass ratio of 1:8 The methanol solution with a mass fraction of 50% was kept for 1~2h, ultrasonicated at 250W for 50min, centrifuged, the supernatant was filtered through a 0.45μm microfiltration membrane, the filtrate was rotary evaporated, concentrated under reduced pressure to obtain a concentrate, and set aside;

[0035] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 450 parts of homogenate, 110 parts of distilled water, 2 parts of 30% hydrochloric acid solution, Stir and mix 4 parts of calcium chloride for 1.7 hours, filter, take the filtrate and add chitosan at a mass ratio of ...

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