Preparation method of eye drops
A technology of eye drops and filtrate, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problems of high toxicity, limited effect, and single drug properties, and achieve muscle relief. Effects of contraction, maintenance, and relief of eye inflammation
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[0021] A preparation method of eye drops, comprising the steps of:
[0022] (1) Take Artemisia annua and add 15% sodium chloride solution at a mass ratio of 1:10, soak it at 20-25°C for 35-45 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and press the mass ratio Add methanol solution with a mass fraction of 50% at 1:8, keep it for 1~2h, ultrasonicate at 250W for 40~60min, centrifuge, take the supernatant and filter it through a 0.45μm microfiltration membrane, take the filtrate to rotary evaporate, and concentrate under reduced pressure to obtain concentrated thing, spare;
[0023] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 400-500 parts of homogenate, 100-120 parts of distilled water, and 2-3 parts by mass. Stir and mix 30% hydrochloric acid solution and 4~5 parts of calcium chloride for 1.5~2 hours, filter, take the fil...
Embodiment 1
[0027] A preparation method of eye drops, comprising the steps of:
[0028] (1) Take Artemisia chinensis and add 15% sodium chloride solution at a mass ratio of 1:10, soak at 20°C for 35 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and add it at a mass ratio of 1:8 The methanol solution with a mass fraction of 50% was kept for 1 h, ultrasonicated at 250W for 40 min, centrifuged, the supernatant was filtered through a 0.45 μm microfiltration membrane, the filtrate was rotary evaporated, concentrated under reduced pressure to obtain a concentrate, and set aside;
[0029] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 400 parts of homogenate, 100 parts of distilled water, 2 parts of 30% hydrochloric acid solution, Stir and mix 4 parts of calcium chloride for 1.5 hours, filter, take the filtrate and add chitosan at a mass ratio o...
Embodiment 2
[0033] A preparation method of eye drops, comprising the steps of:
[0034] (1) Take Artemisia annua and add 15% sodium chloride solution at a mass ratio of 1:10, soak it at 22°C for 40 minutes, take it out and dry it, crush it through an 80-mesh sieve, collect the sieved particles, and add it at a mass ratio of 1:8 The methanol solution with a mass fraction of 50% was kept for 1~2h, ultrasonicated at 250W for 50min, centrifuged, the supernatant was filtered through a 0.45μm microfiltration membrane, the filtrate was rotary evaporated, concentrated under reduced pressure to obtain a concentrate, and set aside;
[0035] (2) Take fresh bovine pancreas and add distilled water to homogenate at a mass ratio of 1:2 to obtain a homogenate. In terms of parts by mass, take 450 parts of homogenate, 110 parts of distilled water, 2 parts of 30% hydrochloric acid solution, Stir and mix 4 parts of calcium chloride for 1.7 hours, filter, take the filtrate and add chitosan at a mass ratio of ...
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