Culture medium and culture method for tissue culture of helianthemum ordosicum

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a culture medium and culture method for tissue culture of helianthemum ordosicum. By taking leaves of adult helianthemum ordosicum plants growing in the wild as an explant for tissue culture to obtain regeneration seedlings, the damage to helianthemum ordosicum wild resources can be greatly reduced, the cost ofmanpower and material resources is reduced, the storage time of experimental materials is prolonged, the utilization rate of the experimental materials is increased, and the reutilization of the experimental materials is gained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture medium and a culture method of Ordos half-helios japonica tissue culture. Background technique [0002] Ordos half-day flower ( Helianthemum ordosicum Y. Z. Zhao, Z. Y. Zhu et R.Cao) is a perennial super-xerophytic small shrub of the family Cistaceae (Cistaceae) and the genus Helianthemum. The area is small and the distribution is concentrated. In 1992, it was written into the Red Book of Plant Protection. C. chinensis grows on low gravel hills with sparse vegetation on the surface. The distribution area is dry and rainless all the year round, and the habitat is very harsh. Its ecological adaptation and evolution mechanism are worthy of study. As an ancient, rare and endangered species, Ordos half-heaven is of great significance to global biodiversity conservation, species origin and evolution, arid area geology and climate and other environmental evolut...

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Problems solved by technology

[0009] 3. Few experimental materials and low yield: the 12-day-old sterile seedlings produced by the seeds stimulated in a sterile environment only contain two cotyledons less than 0.5×0.5cm, and the sterile seedling cotyledons can be used as test materials for explant tissue culture Relatively few, not only that, the proliferation and differentiation ability of the callus after multiple subcultures decreased, and it could no longer produce well-differentiated sterile vaccines

[0010] 4. The cost of materials is high: the cotyledons of sterile seedlings stimulated by seeds are very few raw materials, and the experimental materials cannot be reused. Therefore, the cotyledons of sterile seedlings obtained through seed tissue culture are explant tissue cultures to achieve a large number of multiplications. Collecting the seeds of P. chinensis, and most of them grow on gravel mounds with sparse vegetation on the surface. Multiple collections consume a lot of manpower and economic costs.

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