DsRNA designed based on Orco gene of periplaneta americana, and coding gene, preparation method and application thereof

A technology of Periplaneta americana and genes, applied in the direction of DNA preparation, botanical equipment and methods, DNA/RNA fragments, etc., can solve problems that are only seen in insect aggregation, repulsion, migration, etc., and achieve an environmentally friendly effect

Active Publication Date: 2019-11-19
SOUTH CHINA NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the functional exploration of Orco has mainly focused on basic research fields such as gene identification, gene differentiation, olfactory coding and evolution; the research objects are mostly concentrated in complete metamorphosis insects, such as Dros

Method used

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  • DsRNA designed based on Orco gene of periplaneta americana, and coding gene, preparation method and application thereof
  • DsRNA designed based on Orco gene of periplaneta americana, and coding gene, preparation method and application thereof
  • DsRNA designed based on Orco gene of periplaneta americana, and coding gene, preparation method and application thereof

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Embodiment 2

[0044] Embodiment 2 of the present invention is: a kind of application based on Periplaneta americana Orco gene in the control of Periplaneta americana:

[0045] Conducted in-depth research on the mating behavior of Periplaneta americana with respect to the olfactory receptor Orco. After interfering with the Orco gene, the expression levels of the Orco gene in the antennae, mouthparts and tarsus of the Periplaneta americana were measured to determine the efficiency of interfering with Orco, especially The interference efficiency of the antennal Orco, which is closely related to the sense of smell, and on this basis the mating parameters of the males were examined. In this study, it was shown that reduction of Orco transcript levels significantly delayed the mating time of P. americana males. And further detect the difference in selection preference.

[0046] 1. Live injection of dsRNA that specifically inhibits the Orco gene of Periplaneta americana

[0047] Select healthy m...

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Abstract

The invention discloses dsRNA designed based on an Orco gene of periplaneta americana, and a coding gene, a preparation method and an application thereof. The dsRNA is a double-stranded RNA composed of a nucleotide sequence shown in SEQ ID NO.1 and used as a positive-sense strand and a nucleotide sequence reversely complementary to the nucleotide sequence shown in SEQ ID NO.1 and used as an antisense strand. The dsRNA can be widely applied in preparation of products for preventing and treating the periplaneta americana, interfering the chemical communication of the periplaneta americana or controlling the mating and reproduction of adult periplaneta americana, or preventing and controlling the periplaneta americana and other insects having the same gene target sequence. By blocking key genes of an olfactory system of periplaneta americana, the induction effect of sex pheromone to male insects is inhibited, mating and reproduction of the periplaneta americana can be effectively controlled, and a new technology and strategy are provided for prevention and control of the periplaneta americana.

Description

technical field [0001] The invention relates to the field of sanitary pest control, in particular to a dsRNA designed based on the Periplaneta americana Orco gene, a coding gene, a preparation method and application thereof. Background technique [0002] Cockroaches are a class of important worldwide sanitary pests, causing serious harm to human health and public health. Cockroaches carry a variety of pathogenic microorganisms, which can cause infectious diseases such as cholera, anthrax, and tuberculosis. Cockroach excrement and insect sloughs are also important allergens, which can easily cause allergic reactions such as skin rashes and asthma. Therefore, curbing the reproduction and population expansion of cockroaches from the source has become an important issue that needs to be solved urgently and is becoming urgent in the field of sanitary pest control. [0003] The American cockroach (Periplaneta americana L.) is one of the most common species of cockroaches in the ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/10A01N57/16A01P7/04
CPCA01N57/16C12N15/113
Inventor 李娜李胜黄润
Owner SOUTH CHINA NORMAL UNIVERSITY
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