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Construction method and application of malignant schwannoma xenograft mouse model

A mouse model and xenotransplantation technology, which is applied in the field of establishment of tumor transplantation mouse models, can solve the problems of high failure rate in clinical trials of anti-tumor drugs, achieve the effects of preventing medical social burden, large economic benefits, and improving effectiveness

Active Publication Date: 2019-11-26
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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Problems solved by technology

Studies have found that cells continuously adapt to the in vitro environment during the passage process, and their biological characteristics are inconsistent with tumor cells in vivo. The xenograft model of cell lines is quite consistent with the patient's own tumor, which leads to a high failure rate in clinical trials of anticancer drugs.

Method used

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  • Construction method and application of malignant schwannoma xenograft mouse model
  • Construction method and application of malignant schwannoma xenograft mouse model
  • Construction method and application of malignant schwannoma xenograft mouse model

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Embodiment

[0038] Subcutaneous inoculation: select 10 4-week-old NOD / SCID mice. NOD / SCID mice are non-obese diabetic / severe combined immunodeficiency mice. With a body weight of about 12-15g, soak fresh tumor tissues (or tumor tissues from patients with malignant schwannoma after cryopreservation and resuscitation in liquid nitrogen) in a sterile incubation solution of pure FBS+penicillin and streptomycin (the final concentration of penicillin is 100U / ml, the final concentration of streptomycin is 100U / ml), cut into 1*1*1mm small pieces with sterile scissors. After the mice were anesthetized and fixed, a skin incision was made near the right armpit, the tumor tissue block was implanted subcutaneously (P0), and the surgical incision was sutured. The mice were observed for tumor growth every day. When the tumor grows to a diameter of more than 5mm. Measure the tumor twice a week with a vernier caliper and record the length (a) and width (b) of the tumor, and calculate the tumor volume (V...

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Abstract

The invention provides a construction method and application of a malignant schwannoma xenograft mouse model. The method comprises the steps of firstly, collecting a tumor tissue mass of a malignant schwannoma patient, and then conducting subcutaneous xenograft of the fresh tumor tissue mass of the patient into the body of an immunodeficient mouse under sterile conditions, wherein in the subcutaneous xenograft, the immunodeficient mouse at the age of 2-6 weeks is selected, the gender of the mouse is consistent with that of the patient, the collected fresh tumor tissue mass of the patient is soaked in a sterile incubation solution containing fetal bovine serum, the mouse is fixed after anesthesia, the tumor tissue mass of the patient is implanted into the subcutaneous part of the mouse, anincision is sutured, and the mouse is bred to make the tumor in the body of the mouse grow. The method also involves the orthotopic xenograft of the fresh tumor tissue mass of the patient into the body of the immunodeficient mouse. By means of the method, xenograft mice are used for replacing clinical patients for screening different combination drugs, the most suitable drugs are selected accordingly, the effectiveness is greatly improved, and the side effects of the drugs are greatly reduced.

Description

technical field [0001] The invention relates to the field of establishment of a tumor transplantation mouse model, in particular to a construction method and application of a xenograft mouse model of malignant schwannoma. Background technique [0002] Malignant schwannomas, also known as neurosarcomas, originate from the peripheral nerve sheath and are composed of malignant neuroblasts, most of which are malignant transformations of neurofibromas or schwannomas. It is highly malignant, prone to lung metastasis, extremely high morbidity and mortality, and poor prognosis. Malignant schwannomas have brought a heavy economic burden to the society and even the country, and brought about greater social problems. Because of the heterogeneity of malignant schwannoma, previous basic research results based on malignant schwannoma cell lines can rarely be reproduced in patients with clinical malignant schwannoma. The level of diagnosis and treatment of schwannomas lags behind. [00...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008
Inventor 黎志宏涂超任晓磊陈瑞奇戚麟谢鹏
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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