Method for quickly determining content of xylitol and other polyols

A technology for rapid determination of xylitol, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of low accuracy of results, and achieve the effects of reliable determination results, saving equipment costs, and improving detection efficiency.

Inactive Publication Date: 2019-11-26
ZHEJIANG HUAKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method uses the area normalization method to quantify, and the accuracy of the result is not high

Method used

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  • Method for quickly determining content of xylitol and other polyols
  • Method for quickly determining content of xylitol and other polyols
  • Method for quickly determining content of xylitol and other polyols

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preferred embodiment of the method for rapid determination of xylitol and other polyol content of the present invention, comprises the steps:

[0032] Step 1. Preparation of standard solution stock solution: Dissolve xylitol, mannitol, galactitol, L-arabitol and sorbitol standard products with pyridine, add acetic anhydride, take out the mixed solution after passing through a water bath, let it cool, and then Dilute to volume with acetone to obtain standard solution stock solution.

[0033] Step 2. Preparation of series mixed standard solution stock solutions: select different volumes of standard solution stock solutions and put them into different volumetric flasks, and dilute to the same volume with acetone to obtain series mixed standard solution stock solutions with different concentrations.

[0034] Step 3. Preparation of the test solution: dissolve the xylitol sample with pyridine, add acetic anhydride, take out the xylitol solution after passing through a wat...

Embodiment 2

[0048] The specific embodiment of the method for rapid determination of xylitol and other polyol content of the present invention, comprises the steps:

[0049] 1), preparation of standard solution stock solution,

[0050] Weigh 0.1g of xylitol standard substance, each weigh 20mg of mannitol, galactitol, L-arabitol and sorbitol standard substance, accurate to 0.0001g, into a 20mL volumetric flask, add 1mL of pyridine to dissolve, add 1 mL of acetic anhydride, tightly cap the volumetric flask, take it out in a water bath at 70°C for 30 minutes, let cool, and dilute to the mark with acetone.

[0051] 2), a series of mixed standard solution stock solution,

[0052] Pipette 1.0mL, 2.0mL, 3.0mL, 5.0mL of the standard solution stock solution prepared in step 1) into a 10mL volumetric flask, and dilute to the mark with acetone.

[0053] 3), the preparation of need testing solution,

[0054] Take about 0.5g of xylitol sample into the volumetric flask, accurate to 0.0001g, then add ...

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Abstract

The invention relates to a method for rapidly determining the content of xylitol and other polyols. Pyridine is used to dissolve xylitol and other polyols. Acetic anhydride is added, and then water bath constant temperature heating treatment is performed. The series of mixed standard solutions and a test solution are directly injected into a gas chromatograph for measurement without rotary evaporation. Quantitative analysis is performed through a standard solution external standard method, and the content of xylitol and other polyols is calculated through a formula (A). According to the invention, on the basis of reliable test result of measuring of the content of xylitol and other polyols, the cost of reagents and equipment is saved; measurement time and steps are greatly reduced; and thedetection efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of xylitol determination, in particular to a method for quickly determining the content of xylitol and other polyols. Background technique [0002] Corncobs, bagasse and wood are hydrolyzed and purified to make xylose, which is hydrogenated to obtain food additive xylitol, which is a natural and healthy sweetener. The purity of xylitol is an important indicator to evaluate its quality. The determination methods of xylitol content and other polyols given in the national standard GB1886.234-2016 "National Food Safety Standard Food Additive Xylitol" are liquid chromatography external standard method and gas chromatography internal standard method. details as follows: [0003] Liquid chromatography: Accurately weigh 2.5g of xylitol standard substance and 0.1g each of mannitol standard substance, L-arabitol standard substance, sorbitol standard substance and galactitol standard substance, accurate to 0.0001g, ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/025G01N2030/047
Inventor 陈德水姚智慧徐艳艳程新平徐菁菁石巧余佳江凯莉童湃尤玲梅
Owner ZHEJIANG HUAKANG PHARMA
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