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Solid-phase microextraction-high performance liquid chromatography online detection method for zoledronic acid and risedronic acid

A technology of high performance liquid chromatography and risedronic acid, which is applied in the field of solid-phase microextraction-high performance liquid chromatography on-line combined detection to achieve the effects of improving the probability of action, improving the operating efficiency and enhancing the specific interaction

Active Publication Date: 2021-12-28
福州佳宸生物科技有限公司
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Problems solved by technology

At present, there is no report about the online enrichment and extraction of zoledronic acid and risedronic acid using a HAP functionalized monolithic column

Method used

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  • Solid-phase microextraction-high performance liquid chromatography online detection method for zoledronic acid and risedronic acid
  • Solid-phase microextraction-high performance liquid chromatography online detection method for zoledronic acid and risedronic acid
  • Solid-phase microextraction-high performance liquid chromatography online detection method for zoledronic acid and risedronic acid

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preparation example Construction

[0026] (1) Preparation of matrix monolithic column: wash the empty polytetrafluoroethylene tube with chromatographically pure methanol for half an hour, then place it in a 60°C oven to dry; Formaldehyde solution and 0.1mol / L hydrochloric acid solution were mixed uniformly at a volume ratio of 4:5:1, put into a pre-washed polytetrafluoroethylene tube, and reacted in a 55°C water bath for 10 minutes to prepare a urea-formaldehyde resin matrix monolithic column.

[0027] (2) Coating of polydopamine: Wash the urea-formaldehyde resin matrix monolithic column with water for 1 hour through a microinfusion pump to remove unreacted substances in the column; at the same time, take 5mL of 10mmol / L Tris-HCl solution with pH=8.0 and add 10mg of dopamine , after dissolving and mixing, let it stand for 5 minutes to prepare dopamine solution; then, use a microinfusion pump to fill dopamine solution with urea-formaldehyde resin matrix monolithic column; then, both ends of monolithic column are ...

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Abstract

The invention discloses a solid-phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid. In the present invention, the hydroxyapatite functional monolithic column is firstly prepared by the in-situ mineralization method, and then the monolithic column is used as the solid phase microextraction monolithic column, combined with the solid phase microextraction-high performance liquid chromatography online system, to establish a Solid-phase microextraction-high performance liquid chromatography online detection method for zoledronic acid and risedronic acid. The invention utilizes the specific electrostatic interaction between the hydroxyapatite on the surface of the monolithic column and the P-C-P structure of the analysis object to realize the efficient extraction and enrichment of zoledronic acid and risedronic acid, and eliminate The interference of impurities in the sample matrix on the analytical detection. The method of the invention is simple, the technique is ingenious, the required instruments are highly popular, and it is easy to popularize, and can realize efficient extraction and enrichment of trace amounts of zoledronic acid and risedronic acid in complex actual samples, and meet the relevant high-sensitivity detection requirements.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid. Background technique [0002] Osteoporosis, or osteoporosis, is a metabolic bone disease characterized by a reduction in the amount of bone tissue per unit volume. At present, osteoporosis has climbed to the seventh place in my country's common and frequently-occurring diseases, and the incidence rate is increasing year by year. The situation is not optimistic. According to statistics, there are 88 million people suffering from osteoporosis in my country, with a total prevalence rate of 12.4%. Among them, the prevalence rate of the elderly over 65 years old is over 50%, and the incidence rate of fractures is close to one-third. According to the occurrence of the disease, the drugs clinically used to treat osteoporosis mainl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/08B01D15/22
CPCG01N30/88G01N30/06G01N30/08B01D15/22G01N2030/062G01N2030/8809
Inventor 王家斌戴永伟
Owner 福州佳宸生物科技有限公司
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