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Solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid

A technology of high-performance liquid chromatography and risedronic acid, which is applied in the field of solid-phase microextraction-high performance liquid chromatography on-line combined detection, to achieve the effects of cleaning and regeneration, prolonging service life, and enhancing specific interaction

Active Publication Date: 2019-12-13
福州佳宸生物科技有限公司
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Problems solved by technology

At present, there is no report about the online enrichment and extraction of zoledronic acid and risedronic acid using a HAP functionalized monolithic column

Method used

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  • Solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid
  • Solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid
  • Solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid

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preparation example Construction

[0026] (1) Preparation of matrix monolithic column: wash the empty polytetrafluoroethylene tube with chromatographically pure methanol for half an hour, then place it in a 60°C oven to dry; Formaldehyde solution and 0.1mol / L hydrochloric acid solution were mixed uniformly at a volume ratio of 4:5:1, put into a pre-washed polytetrafluoroethylene tube, and reacted in a 55°C water bath for 10 minutes to prepare a urea-formaldehyde resin matrix monolithic column.

[0027] (2) Coating of polydopamine: Wash the urea-formaldehyde resin matrix monolithic column with water for 1 hour through a microinfusion pump to remove unreacted substances in the column; at the same time, take 5mL of 10mmol / L Tris-HCl solution with pH=8.0 and add 10mg of dopamine , after dissolving and mixing, let it stand for 5 minutes to prepare dopamine solution; then, use a microinfusion pump to fill dopamine solution with urea-formaldehyde resin matrix monolithic column; then, both ends of monolithic column are ...

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Abstract

The invention discloses a solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid. First, a hydroxyapatite functionalized monolithic column is prepared by means of in-situ mineralization, and then, a solid-phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid is established by taking the monolithic column as a solid phase microextraction monolithic column and adopting a solid phase microextraction-high performance liquid chromatography online combined system. Efficient extraction and enrichment of zoledronic acid and risedronic acid are achieved through specific electrostatic interaction between hydroxyapatite on the surface ofthe monolithic column and a P-C-P structure carried by an analysis object, and the interference of impurities in a sample matrix on analysis and detection is eliminated. The method is simple, ingenious in process, high in popularization degree of required instruments and easy to popularize, can achieve efficient extraction and enrichment of trace zoledronic acid and risedronic acid in complex actual samples, and meets related high-sensitivity detection requirements.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a solid phase microextraction-high performance liquid chromatography online combined detection method for zoledronic acid and risedronic acid. Background technique [0002] Osteoporosis, or osteoporosis, is a metabolic bone disease characterized by a reduction in the amount of bone tissue per unit volume. At present, osteoporosis has climbed to the seventh place in my country's common and frequently-occurring diseases, and the incidence rate is increasing year by year. The situation is not optimistic. According to statistics, there are 88 million people suffering from osteoporosis in my country, with a total prevalence rate of 12.4%. Among them, the prevalence rate of the elderly over 65 years old is over 50%, and the incidence rate of fractures is close to one-third. According to the occurrence of the disease, the drugs clinically used to treat osteoporosis mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/08B01D15/22
CPCG01N30/88G01N30/06G01N30/08B01D15/22G01N2030/062G01N2030/8809
Inventor 王家斌戴永伟
Owner 福州佳宸生物科技有限公司
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