Application of 2,5-furan dimethanol in preparing antitumor drugs
An anti-tumor drug, a technology of furandimethanol, which is applied in the field of medicine, can solve problems such as none, and achieve the effects of less toxic and side effects, mild toxic and side effects, and low difficulty in obtaining
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[0023] Example 1
[0024] Cytotoxicity test of 2,5-furandimethanol on human neuroblastoma SH-SY5Y:
[0025] SH-SY5Y was purchased from the National Laboratory Cell Resource Sharing Service Platform (3111C0001CCC000026).
[0026] Complete medium: 100 mL complete medium contains 89.1 mL RPMI-1640 medium, 9.9 mL fetal bovine serum and 1 mL penicillin-streptomycin solution. In the penicillin-streptomycin solution, the content of penicillin is 10KU / mL, and the concentration of streptomycin is 10mg / mL.
[0027] Change the medium: Use a pipette to remove the complete medium in the culture flask, wash the cells 2-3 times with 2-3 mL of PBS solution (phosphate buffer solution), add 4 mL of fresh complete medium, and place at 37°C, CO 2 Continue to culture in a 5% cell incubator.
[0028] Passaging: Use a pipette to remove the medium in the culture flask, wash the cells 2-3 times with 2-3 mL PBS solution, add 500 μL trypsin containing 0.25% EDTA for 1-2 min, discard the trypsin; add 3 mL complet...
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[0037] Example 2
[0038] Cytotoxicity test of 2,5-furandimethanol on human lung cancer cell A549:
[0039] A549 was purchased from the National Laboratory Cell Resource Sharing Service Platform (3111C0001CCC000002).
[0040] Complete medium: 100 mL complete medium contains 89.1 mL RPMI-1640 medium, 9.9 mL fetal bovine serum and 1 mL penicillin-streptomycin solution.
[0041] Change the medium: Use a pipette to remove the culture medium in the culture flask, wash the cells 2-3 times with 2-3mL PBS solution, add 4mL fresh complete medium, and keep at 37℃, CO 2 Continue to culture in a 5% cell incubator.
[0042] Passaging: Use a pipette to remove the medium in the culture flask, wash the cells 2-3 times with 2-3mL PBS solution, add 500μL trypsin containing 0.25% EDTA for 1-2min, discard the trypsin, and add 3mL complete medium Stop the digestion, mix by pipetting, keep 1mL of liquid in the culture flask and supplement with 3mL of fresh complete medium, at 37℃, CO 2 Continue to culture i...
Example Embodiment
[0052] Example 3
[0053] Cytotoxicity test of 2,5-furandimethanol on human gastric adenocarcinoma cell SGC7901:
[0054] SGC7901 was purchased from the National Laboratory Cell Resource Sharing Service Platform (3111C0001CCC000236).
[0055] Complete medium: 100 mL complete medium contains 89.1 mL RPMI-1640 medium, 9.9 mL fetal bovine serum and 1 mL penicillin-streptomycin solution.
[0056] Change the medium: use a pipette to remove the culture medium in the culture flask, wash the cells 2-3 times with 2-3mL PBS solution, add 4mL fresh complete medium at 37℃, CO 2 Continue to culture in a 5% cell incubator.
[0057] Passaging: Use a pipette to remove the medium in the culture flask, wash the cells 2-3 times with 2-3mL PBS solution, add 500μL trypsin containing 0.25% EDTA for 1-2min, discard the trypsin, and add 3mL complete medium Stop the digestion, mix by pipetting, keep 1mL of liquid in the culture flask and supplement with 3mL of fresh complete medium at 37℃, CO 2 Continue to cul...
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