Preparation method of duck natural immune enhancer based on traditional Chinese medicine extract

Abstract

The present invention discloses a preparation method of a duck natural immune enhancer based on a traditional Chinese medicine extract. The preparation method comprises the following steps: (1) establishment of a rutin standard curve; (2) extraction of astragalus membranaceus flavonoids; and (3) calculation of astragalus membranaceus flavonoid concentration. The provided duck natural immune enhancer based on the traditional Chinese medicine extract can significantly increase levels of duck serum cytokines of IL-1-beta, IFN-alpha and IFN-beta, up-regulates tissue expression levels of natural immune-related genes RIG-I, TLR3, TLR7, IFN-alpha, IFN-beta, IL-1-beta and TNF-alpha, inflammation-related genes COX2 and iNOS, and cell apoptosis-related gene Caspase3 in duck immune organs of liver, spleen, thymus and bursa of fabricius, and enhances duck natural immune response and disease resistance performance.

Description

technical field

[0001] The invention belongs to the technical field of agriculture and relates to a preparation method of a duck natural immune enhancer based on traditional Chinese medicine extracts. Background technique

[0002] With the rapid development of large-scale and intensive breeding methods in my country's animal husbandry, the hazards of animal diseases are becoming more and more serious, which has become the biggest obstacle to the healthy development of my country's animal husbandry. At present, immunoprevention and drug therapy are the main means of preventing and controlling animal diseases. Although they have played an important role, in recent years, with the increasing awareness of drug residues in animal products and environmental protection, the traditional methods of animal disease prevention and control have been greatly affected. These challenges have prompted people to continuously explore new prevention and control technologies.

[0003] The immun...

Examples

Embodiment Construction

[0017] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0018] 1. Preparation of duck natural immune enhancer

[0019] Extracting and identifying pharmacologically active ingredients from a certain traditional Chinese medicine with clear chemical composition and pharmacological efficacy, and is used to prepare duck natural immune enhancer.

[0020] 2. Experimental animal grouping and treatment

[0021] The 1-day-old ducklings hatched from the same batch were selected, and after 7 days of adaptive feeding, they were randomly divided into a test group and a control group, with 20 ducklings in each group. The ducklings in the test group were subcutaneously injected with a synthetic duck natural immune enhancer (2.0 mg / feather), and the ducklings in the control group were subcutaneously injected with the same volume of normal saline for 3 consecutive days. During the experiment, ducklings in ea...

technical field

[0001] The invention belongs to the technical field of agriculture and relates to a preparation method of a duck natural immune enhancer based on traditional Chinese medicine extracts. Background technique

[0002] With the rapid development of large-scale and intensive breeding methods in my country's animal husbandry, the hazards of animal diseases are becoming more and more serious, which has become the biggest obstacle to the healthy development of my country's animal husbandry. At present, immunoprevention and drug therapy are the main means of preventing and controlling animal diseases. Although they have played an important role, in recent years, with the increasing awareness of drug residues in animal products and environmental protection, the traditional methods of animal disease prevention and control have been greatly affected. These challenges have prompted people to continuously explore new prevention and control technologies.

[0003] The immun...

Examples

Embodiment Construction

[0017] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0018] 1. Preparation of duck natural immune enhancer

[0019] Extracting and identifying pharmacologically active ingredients from a certain traditional Chinese medicine with clear chemical composition and pharmacological efficacy, and is used to prepare duck natural immune enhancer.

[0020] 2. Experimental animal grouping and treatment

[0021] The 1-day-old ducklings hatched from the same batch were selected, and after 7 days of adaptive feeding, they were randomly divided into a test group and a control group, with 20 ducklings in each group. The ducklings in the test group were subcutaneously injected with a synthetic duck natural immune enhancer (2.0 mg / feather), and the ducklings in the control group were subcutaneously injected with the same volume of normal saline for 3 consecutive days. During the experiment, ducklings in ea...

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