A method for microbial pretreatment to promote composting of livestock and poultry manure
A livestock and poultry manure pretreatment technology, applied in the direction of microbial treatment, joint biological treatment, biological organic part treatment, etc., to achieve the effect of simple operation, increased abundance, and low cost
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Embodiment 1
[0038] The preparation of the liquid mycophyllum compact comprises the following steps:
[0039] (1) Activation culture: inoculate Glucophyllum compactus on a solid medium, and culture it statically at 28° C. for 5 days;
[0040] (2) Seed culture: wash the Mycophyllum thallus on the solid medium in step (1) with sterile physiological saline, vortex and oscillate for 5 minutes to obtain a thalline suspension, inoculate into 100ml liquid culture medium, and cultivate The conditions are 28°C shaking culture for 24 hours, the shaking speed is 200rpm, and the seed liquid is obtained;
[0041] (3) Expanded cultivation: inoculate the seed solution obtained in step (2) into 1L liquid culture solution by volume ratio of 10%, and carry out expanded cultivation. The cultivation condition is 28° C. shaking table shaking culture for 48 to 72 hours, and the shaking speed is 200 rpm , and then the obtained liquid inoculum of Glycophyllum compacta was stored at 4°C until use. The content of...
Embodiment 3
[0057] The present embodiment is comparative example, compost raw material composition and method and measuring method are all identical with embodiment 2, but omit microbial pretreatment step (being step (1) and step (2)) in embodiment 2, directly carry out conventional composting as Control group, with embodiment 2 as experimental group, compare the properties of wheat straw before and after pretreatment, and material temperature change, seed germination index, CO 2 Emissions, humus content and microbial populations.
[0058] The basic properties of this experimental example compost raw material are as follows in Table 1:
[0059] Table 1 Basic properties of compost raw materials
[0060]
[0061] Experimental results:
[0062] 1. Liquid chromatography-mass spectrometry (LC-MS) was used to determine the contents of various phenolic substances before and after pretreatment of wheat straw by Glyophyllum compactus. The results are shown in Table 2.
[0063] Table 2 Phenol...
Embodiment 4
[0088] This embodiment also uses the liquid bacterial agent of Myxophyllum compactus in Example 1 to carry out the method for rice straw pretreatment to promote composting of livestock and poultry manure, and the experimental group includes the following steps:
[0089] (1) Pretreatment: Add urea at a mass ratio of 2% to 10 kg of rice straw crushed to a length of 1 to 3 cm, adjust the water content to 80%, and the pH value to 7.5. By 4% mass ratio, the liquid inoculum of Myxophyllum compactus obtained in Example 1 was inoculated into 10 kg of rice straw, fully stirred, and packed into a 50L plastic fermentation tank for solid-state fermentation. The fermentation time was 5 days, and the fermentation temperature was 28° C.;
[0090] (2) Stockpiling: after the pretreatment, mix the pretreated straw and fresh chicken manure with a moisture content of 72% in a mass ratio of 1:5 using manual or mechanical methods, and adjust the moisture content to 55%. / N ratio is 25;
[0091] (3...
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