Simultaneous and rapid determination method of vitamin A, vitamin D3 and vitamin E

A vitamin and solution technology, which is applied in the field of analysis and detection, can solve the problems of shortening and inability to carry out at the same time, and achieve the effects of avoiding retesting, shortening preprocessing time, and avoiding test errors

Inactive Publication Date: 2019-12-31
通标标准技术服务(青岛)有限公司
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that vitamin A and vitamin D cannot be produced simultaneously in the prior art. 3 and vitamin E content detection, a simultaneous and rapid detection of vitamin ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Simultaneous and rapid determination method of vitamin A, vitamin D3 and vitamin E
  • Simultaneous and rapid determination method of vitamin A, vitamin D3 and vitamin E
  • Simultaneous and rapid determination method of vitamin A, vitamin D3 and vitamin E

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Vitamin A, Vitamin D in health food 3 and vitamin E content detection

[0035] 1. Reagents and materials:

[0036] Methanol: chromatographically pure;

[0037] Dimethyl sulfoxide: chromatographically pure;

[0038] n-Hexane: chromatographically pure or analytically pure;

[0039] Vitamin A acetate standard: CAS: 127-47-9, purity not less than 99.0%;

[0040] Vitamin D 3 Standard product: CAS: 67-97-0, purity not less than 99.0%;

[0041]DL-а-tocopheryl acetate standard product: CAS: 7695-91-2, purity not less than 99.0%.

[0042] 2. Standard stock solution:

[0043] Preparation of vitamin A standard stock solution (1mg / mL): Accurately weigh 50mg (accurate to 0.01mg) vitamin A acetate standard substance in a 50mL brown volumetric flask, dissolve with methanol to volume; vitamin D 3 Preparation of standard stock solution (1mg / mL): Accurately weigh 50mg (accurate to 0.01mg) of vitamin D 3 Dissolve the standard product in a 50mL brown volumetric flask and dilute to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention belongs to the technical field of analysis and detection, in particular to a simultaneous and rapid determination method of vitamin A, vitamin D<3> and vitamin E. The method comprises the following steps: (1) vitamin A, vitamin D<3> and vitamin E in a sample to be tested are extracted with dimethyl sulfoxide; and the obtained extraction liquid is oscillated and extracted withn-hexane, concentrated and redissolved to obtain a sample solution; (2) standard solutions of vitamin A, vitamin D<3> and vitamin E are prepared; and multiple standard series of solutions are prepared according to the concentration gradient; (3) the sample solution and the standard solutions are detected and analyzed with the high performance liquid chromatography; and the contents of vitamin A,vitamin D<3> and vitamin E are determined with an external standard method. With the method, simultaneous determination of vitamin A, vitamin D<3> and vitamin E is realized; the pretreatment time of the sample is greatly shortened; the work efficiency is improved; at the same time, the detection accuracy is improved; and the laboratory cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, in particular to a method for synchronous and rapid detection of vitamin A and vitamin D 3 and vitamin E methods. Background technique [0002] Vitamins A, D and E are fat-soluble vitamins, and fat-soluble vitamins are polypentadienyl compounds composed of long hydrocarbon chains or fused rings. Vitamin A (vitamin A acetate), vitamin D 3 (cholecalciferol) and vitamin E (vitamin E acetate) are essential for human health, among them, vitamin D 3 It is an important nutritional ingredient for mammals, and it can promote calcium and phosphorus metabolism and bone formation in mammals. Therefore, vitamin A and vitamin D 3 Vitamin E and vitamin E are the main nutritional components of infant milk powder and rice flour, and are also important components of various health foods. [0003] Vitamin A and vitamin E have strong ultraviolet absorption and high content in food. One-dimensional...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/60G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/6052G01N30/74
Inventor 吕明春季爱云黄翠玲曹俊伟李传慧殷航
Owner 通标标准技术服务(青岛)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap